李杨钧　章　维.宁波市第二医院肝胆、微创外科，浙江宁波　3500；.宁波市第二医院消化科，浙江宁波　3500

Survivin与Caspase-3在胆管细胞癌中的表达及相关性研究

李杨钧1章维2
1.宁波市第二医院肝胆、微创外科，浙江宁波315010；2.宁波市第二医院消化科，浙江宁波315010

目的探讨Survivin与Caspase-3在胆管细胞癌中的表达，并探讨二者之间的相关性。方法选择2013年12月～2015年12月在我院手术治疗的胆管细胞癌患者40例（A组）手术标本作为研究对象，同时取癌旁胆管组织30例（B组）。另外选取同期手术的10例正常组织（肝血管瘤旁正常肝内胆管组织或者肝外伤后切除肝组织的正常肝内胆管组织）作为对照（C组）。比较各组Survivin与Caspase-3表达的阳性率与临床病理参数的关系，及Survivin在胆管细胞癌中的表达与Caspase-3的相关性。结果Survivin与Caspase-3在胆管细胞癌中的阳性率分别为75.0%、40.0%，其中Survivin在A组中的阳性率显著高于B组、C组，差异有统计学意义（P＜0.05）。而Caspase-3在A组中的阳性率低于B组、C组，差异有统计学意义（P＜0.05）。Survivin、Caspase-3在胆管细胞癌中的表达与分化程度及淋巴结转移有密切关系，差异有统计学意义（P＜0.05）。胆管细胞癌中Survivin阳性30例，Caspase-3阳性16例，二者均为阳性7例，二者均为阴性1例，应用相关分析显示，Survivin在胆管细胞癌中的表达与Caspase-3呈负相关（r=-0.723，P＜0.05）。结论Survivin在胆管细胞癌中的阳性表达率较高，但Caspase-3较低，二者呈负相关，考虑可能是Survivin抑制了Caspase-3介导的细胞凋亡，对Survivin与Caspase-3的研究有助于深入了解胆管细胞癌的发生、发展机制。

胆管细胞癌；Survivin；Caspase-3；相关性

［Abstract］Objective To investigate the expression of Survivin and Caspase-3 in cho1angiocarcinoma and to exp1ore the corre1ation between the two.Methods 40 cases（group A）with cases of bi1e duct carcinoma were se1ected as the study subjects，and 30 cases（group B）were co11ected from the surgica1 treatment of cho1angiocarcinoma in our hospita1 during December 2013 to December 2015.In addition，10 cases of norma1 1iver tissue（norma1 intrahepatic bi1e duct tissue or 1iver injury after 1iver trauma）were se1ected as contro1 group（group C）.The re1ationship between the expression of Survivin and in each group，the re1ationship between the positive rate of Caspase-3 expression and the c1inicopatho-1ogica1 parameters，and the corre1ation between the expression of Survivin and the expression of Caspase-3 in cho1angiocarcinoma were compared.Results The positive rates of Survivin and Caspase-3 in cho1angiocarcinoma were 75.0% and 40.0%，respective1y.The positive rate of Survivin in group A was significant1y higher than that in group B and group C（P＜0.05）.And the positive rate of Caspase-3 in group A was 1ower than that of group B and group C（P＜0.05）. The expression of Caspase-3 and Survivin was c1ose1y re1ated to the degree of differentiation and 1ymph node metastasis，and the difference was statistica11y significant（P＜0.05）.Bi1e duct ce11 carcinoma survivin in 30 cases of positive，caspase-3 was positive in 16 cases，both were positive in 7 cases，two were negative in 1 case，the app1ication corre1ation ana1ysis showed that survivin in bi1e duct carcinoma and the expression of Caspase-3 was negative1y corre1ated （r=-0.723，P＜0.05）.Conclusion The positive expression of Survivin in ce11s of bi1e duct cancer rate is high，but caspase-3 is 1ow，the second is negative1y re1ated，consider possib1e is by inhibition of caspase-3 apoptosis mediated by Survivin.The research of Survivin and Caspase-3 he1ps to understanding the deve1opment mechanism of cho1ahgiocarcinoma.

［Key words］Cho1angiocarcinoma；Survivin；Caspase-3；Corre1ation

胆管细胞癌（cho1angiocarcinoma）是常见的消化系统恶性肿瘤之一，近年来发病率逐年升高。胆管细胞癌的发生发展与其他肿瘤一样，其发生发展是多因素、涉及多基因改变和多阶段致病的复杂过程［1］。……