王　盼　单　伟辽宁医学院，辽宁锦州　121000

局部联合移植hUCMSCs与VEGF对MCAO大鼠神经功能的影响

王盼单伟▲
辽宁医学院，辽宁锦州121000

目的将血管内皮生长因子（vascu1ar endothe1ia1 growth factor，VEGF）与体外培养的人脐带间充质干细胞（human umbi1ica1 cord derived mesenchyma1 stem ce11s，hUCMSCs）联合植入脑缺血（midd1e cerebra1 artery occ1usion，MCAO）模型大鼠脑内，观察神经功能改善情况。 方法选择75只SD大鼠，实验分组如下：MCAO组，仅制备缺血模型；假手术组，仅分离颈总动脉，不栓塞；VEGF组，缺血24 h后，局部缺血区给予VEGF治疗；hUCMSCs组，缺血24 h后，局部缺血区给予hUCMSCs治疗；hUCMSCs+VEGF联合组，缺血24 h后，局部缺血区给予hUCMSCs+ VEGF治疗。观察缺血前、缺血后1 d、4 d、7 d、14 d、21 d各个时间点改良神经功能评分（mNSS）变化。结果hUCMSCs的增殖及分化不受Brdu标记的影响；缺血后，随着治疗时间的延续，单因素治疗组内（VEGF组、hUCMSCs组）大鼠的mNSS评分减低，联合组治疗效果优于单独因素处理组，且在不同时间点之间有显著性差异（F= 5.018，P＜0.01）。MCAO组的mNSS评分高于VEGF组、hUCMSCs组和联合组，差异有统计学意义（P＜0.05），在所选择观察的6个时间点内，联合组21 d时mNSS评分得分最低。结论VEGF联合hUCMSCs移植入MCAO模型大鼠脑内，可促进缺血模型大鼠的神经功能恢复。

脑缺血；脐带间充质干细胞；血管内皮细胞生长因子；神经功能

［Abstract］Objective To imp1ant vascu1ar endothe1ia1 growth factor（VEGF）and human umbi1ica1 cord derived mesenchyma1 stem ce11s（hUCMSCs）together into the brain of mode1 rats with midd1e cerebra1 artery occ1usion（MCAO）and observe the nerve function improvement situation.Methods Seventy-five SD rates were divided into the fo11owing groups:MCAO group，in which on1y ischemia mode1 was estab1ished，fake surgery group，in which on1y common carotid artery was separated without embo1ism，VEGF group，in which VEGF treatment was given in 1oca1 ischemic region 24 hours after ischemia，hUCMSCs group，in which hUCMSCs treatment was given in 1oca1 ischemic region 24 hours after ischemia，and hUCMSCs+VEGF combination group，in which hUCMSCs+VEGF treatment was given in 1oca1 ischemic region 24 hours after ischemia.The changes of modified neuro1ogica1 severity scores（mNSS）before ischemia and 1 day，4 days，7 days，14 days and 21 days after ischemia were observed.Results Pro1iferation and differentiation of hUCMSCs were not inf1uenced by Brdu mark.After ischemia，as treatment continued，mNSS scores of the sing1e factor treatment groups（VEGF group and hUCMSCs group）reduced，and combined treatment group was better than sing1e factor treatment group in treatment effects，with significant differences at different time points（F=5.018，P＜0.01）.Compared with the VEGF group，hUCMSCs group and combined group，the score of mNSS of the MCAO group was higher，and the difference was statistica11y significant（P＜0.05），score of mNSS in the combined group showed the 1owest scoring at the 21 d，in the se1ected six time point.Conclusion Combined transp1antation of VEGF and hUCMSCs into brain of MCAO mode1 rates can promote the nerve function recovery of ischemic mode1 rats.

［Key words］Cerebra1 ischemia；Umbi1ica1 cord mesenchyma1 stem ce11s；Vascu1ar endothe1ia1 growth factor；Nerve function

脑缺血是临床上神经系统疾病中较为常见，并且在临床上有着高发病率、高致残率、高死亡率的三大显著特点，尤其是对老年人群的危害性更为明显［1，2］，这也就要求我们研究者给予更多的关注。今年来随着科技的不断进步，越来越多的实验已经证实干细胞对于脑缺血可以发挥神经保护作用，从不同程度上可以缓解脑组织急性缺血所带来的损伤。……