刘珺，廖剑绚，唐洪波，周芝芳南华大学附属第一医院耳鼻喉科，湖南衡阳　421001

花姜酮对鼻咽癌细胞株放疗敏感性的影响

刘珺，廖剑绚，唐洪波，周芝芳
南华大学附属第一医院耳鼻喉科，湖南衡阳421001

目的观察花姜酮处理对鼻咽癌CNE-2细胞放疗敏感性的影响。方法体外培养鼻咽癌细胞系CNE-2。并根据不同的实验目的分为对照组、γ-射线干预组以及γ-射线联合花姜酮处理组。其中对照组包括空白对照组和花姜酮处理组，分别给予等体积培养基或0～20μmol/L花姜酮处理48 h，γ-射线组采用剂量率为100cGy的γ-射线照射，联合治疗组细胞采用花姜酮预处理24 h后用γ-射线照射。处理结束后采用MTT法检测CNE-2细胞增殖情况，流式细胞术检测CNE-2细胞周期改变情况。结果 0～20μmol/L花姜酮或100cGy γ-射线单独处理48 h后，对人鼻咽癌CNE-2细胞生长无明显影响；而同时联合不同浓度花姜酮与100cGy的γ-射线处理后，随着花姜酮浓度的增高，细胞增殖水平随之降低。流式细胞术也显示，0～20μmol/L花姜酮联合100cGy γ-射线照射后，可将细胞阻滞在G1期，同时可降低S期、G2/M期细胞的比例。结论花姜酮通过改变CNE-2细胞周期而发挥对细胞放疗增敏作用。

花姜酮；鼻咽癌细胞；放疗；敏感性

［Abstract］Objective To observe the effect of zerumbone treatment on the radiosensitivity of the nasopharyngeal carcinoma CNE-2 cells.Methods nasopharyngeal cancer cell line CNE-2 was cultured in vitro and divided into the control group，gamma ray intervention group and gamma ray combined with zerumbone treatment group，among them，the control group included the blank control group and the zerumbone treatment group，and they were respectively given equal-volume culture media or 0-20μmol/L zerumbone treatment for 48h，the gamma ray group adopted 100cGy gamma ray radiation，the combined treatment group adopted gamma ray radiation after the zerumbone pretreatment for 24h，and the CNE-2 cell proliferation was detected by MTT method and CNE-2 cell cycle changes were detected by flow cytometry at the end of treatment. Results After 48h single treatment，0-20μmol/L zerumbone or 100cGy gamma ray radiation had no obvious effect on the human nasopharyngeal carcinoma CNE-2 cell growth；but the combined treatment of different concentrations of zerumbone and 100cGy gamma ray showed that with the increase of the zerumbone concentration，the cell proliferative level decreased，and the flow cytometry also showed that after the treatment of 0-20μmol/L zerumbone and 100cGy gamma ray radiation，the cells were arrested in G1 phase，at the same time，the cell proportions in S stage and G2/M stage could be reduced.Conclusion Zerumbone plays a sensibilization role in cell radiotherapy by changing the CNE-2 cycle.

［Key words］Zerumbone；Nasopharyngeal cancer cell；Radiotherapy；Sensitivity

鼻咽癌（nasophary ngeal carcinoma，NPC）是我国两广地区最常见的头颈部恶性肿瘤之一，病理学上大部分表现为低分化鳞癌和未分化癌，且具有高转移和高复发性［1］。NPC病因尚未明确，吸烟、EB病毒感染、饮食与居住环境受均与NPC的发病有关［2］。尽管放射治疗可提高NPC的局部控制率，但其应用受到多因素的限制［3］。研究表明，某些中草药提取物对提高肿瘤细胞对放疗的敏感性方面具有协同作用，花姜酮就是其中一种［4-5］。……