秦培洁　　王柳青　　程志立　　狄　颖　　刻剑锋

中国中医科学院中国医史文献研究所，北京　100700

应激性胃溃疡大鼠前爪不同区域黑色素变化的ghrelin-POMC通路研究

秦培洁王柳青程志立狄颖刻剑锋▲

中国中医科学院中国医史文献研究所，北京100700

目的探讨应激性胃溃疡大鼠前爪手掌不同区域黑色素变化及其机制。 方法采用随机数字表法将20只SD雄性大鼠分为空白对照组、模型组，每组各10只，利用无水乙醇灌胃复制应激性胃溃疡模型后，利用多巴氧化酶检测两组大鼠前爪皮肤黑色素变化；用ELISA法检测两组大鼠血清ghre1in水平；用RT-PCR法检测两组大鼠胃黏膜组织ghre1in mRNA、垂体和前爪皮肤组织POMC mRNA水平。结果模型组大鼠5区的黑色素细胞明显增多；与空白对照大鼠比较，模型组大鼠的血清ghre1in水平及ghre1in mRNA降低，且差异有统计学差异（P＜0.05）；模型组大鼠的垂体和前爪皮肤组织POMC mRNA水平显著高于空白对照组（P＜0.05）。结论应激性胃溃疡大鼠的病理变化可以在手掌特定区域通过黑色素的变化反映出来，初步推测其内在机制可能是通过作用于ghre1in-POMC通路实现的。

手诊；大鼠前爪；黑色素含量；脑肠肽；阿黑皮素原

［Abstract］Objective To investigate the change and the mechanism of me1anin in forepaw skin of rats with irritab1e u1cer.Methods 20 rats were divided into two groups by random number tab1e∶b1ank contro1 group and mode1 group，each group had 10 rats.Acute gastric mucosa1 injury mode1 was estab1ished by intragastric administration of 75% ethano1.The me1anin 1eve1s of forepaw skin in the two groups were detected by dopa oxidase.Serum ghre1in 1eve1s in the two groups were detected by ELISA method.Ghre1in mRNA of Gastric mucous tissue，POMC mRNA of pituitary and forepaw skin tissues were detected by RT-PCR method.Results The me1anin in 5-zone were significant1y increased in the mode1 group.Compared with the b1ank contro1 group，the ghre1in 1eve1 and ghre1in mRNA in the mode1 group significant1y decreased（P＜0.05）.The POMC mRNA 1eve1s in the pituitary and forepaw skin tissues of the mode1 group were significant1y higher than those in the b1ank contro1 group（P＜0.05）.Conclusion The patho1ogica1 changes of rats with irritab1e u1cer can be ref1ected by me1anin in particu1ar area of forepaw，pre1iminary specu1ation that the interna1 mechanism may be rea1ized by ghre1in-POMC pathway.

［Key words］Hand diagnosis；Rat forepaw；Me1anin content；Ghre1in；POMC

气色形态手诊是由刘剑锋教授首次提出的创新中医诊断方法，是在中医望色诊病理论基础上结合临床实践建立起来的。在经过多年临床实证的基础上，近年来课题组致力于寻找气色形态手诊的机制，以期为中医“司内揣外”理论提供研究方法和实验依据。在前期的工作中，课题组发现急性胃损伤大鼠的前爪特定部位皮肤黑色素细胞增多，本研究在前期研究基础上探讨其内在机制。……