杨晓泉 郭永强 夏从龙

[摘要]“蒂达”是藏药中治疗肝胆疾病最具有代表性的常用药物之一，比较藏药“蒂达”基原植物中7种有效成分的含量，并进行整合评控，为其资源利用提供了科学依据。该文采用HPLCDAD扫描3D图谱，直接获得各环烯醚萜类、口山酮及齐墩果酸的最适宜吸收波长。根据结果开启210， 240， 265 nm多波长采集数据同时检测7种有效成分。通过分析计算，7种成分在线性范围内线性关系良好，相关系数r均达到0999 9，平均回收率99%～101%，RSD均小于3%。对结果进行归一化与三维数据集成化，获得各种植物化学品质综合指数。上述结果表明藏药“蒂达”基原植物中绝大多数含有这7种有效成分，但种间差异较大，通过化学品质综合指数进行整合评控对质量控制有参考价值。

[关键词]“蒂达”；HPLCDAD；多波长检测；化学品质综合指数；整合评控

[Abstract]"Dida" is one of the most representative commonly used Tibetan medicines in treatment of hepatobilaiary diseases To analyze and compare the content of 7 effective constituents in 11 species which are used as Dida Integrated evaluation of them， which supply a basis for the resources exploitation for them In this paper， most appropriate absorption wavelengths of different natural iridoid compounds， xanthones and oleanolic acid have been performed by using 3D spectrum technique According to the results， the wavelength 210， 240 and 265 nm were used for detection of 7 effective constituents By means of calculation， determination of 7 effective constituents indicated good linearity over the linear range with coefficients （r） of 0999 9 respectively The recoveries were found in the average range of 99% to 101% with RSD being less than 3% Normalize and integrate by threedimension the data Get their integrated chemical quality index（ICQI） Results suggested that these plants used as Dida in Tibet medicine mostly contain these 7 effective constituents， but the different is notable The integrated evaluation by the integrated chemical quality index （ICQI） has reference value for quality control

[Key words]Dida； HPLCDAD； multiple wavelength detection； integrated chemical quality index（ICQI）； integrated evaluation

doi：10.4268/cjcmm20160919

“蒂达”是藏药中治疗肝胆疾病最具有代表性的常用药物之一，具有悠久的用药历史，它指“味苦，能治疗肝胆疾病”的一类功效相近的药物的“总称”，《晶珠本草》[1]记载：“蒂达可清热，治胆病、血病，有清肝利胆、退诸热的作用。”其基原植物统称为“藏茵陈”，在云南、四川、西藏等地分布极广。沈黎明等[2]整理蒂达在云南分布，认定青叶胆Swertia mileensis，紫红獐牙菜S punicea，丽江獐牙菜S delavayi，椭圆叶花锚H elliptica等作为藏茵陈使用；姜海等[3]调查蒂达在四川分布，认定川西獐牙菜S mussotii，圈纹獐牙菜S cincta，大籽獐牙菜S macrosperma为藏药蒂达基原植物；钟国跃等[4]整理藏药蒂达品种，认定其基原植物涉及龙胆科獐牙菜属Swertia，花锚属Halenia，扁蕾属Gentianopsis及虎耳草科虎耳草属Saxifraga等，其中青叶胆S mileensis自1975年以后均被收载于《中国药典》。……