马开明，王耀辉，吴　铭，樊　航，闫钰鑫，蔡　欢，牛利娜，李　丹，王国英*

(1. 河南大学医学院 细胞与分子免疫学实验室, 河南 开封 475004；2. 河南大学 医学院，河南 开封 475004)

四种旋毛虫肌幼虫囊包染色方法的比较探讨

马开明1，王耀辉1，吴铭2，樊航2，闫钰鑫2，蔡欢2，牛利娜2，李丹2，王国英1*

(1. 河南大学医学院 细胞与分子免疫学实验室, 河南 开封 475004；2. 河南大学 医学院，河南 开封 475004)

摘要：目的比较4种染色方法制作旋毛虫肌幼虫囊包标本的效果。 方法①醋酸卡红(含铁)染色液染色：染色1.5 h，不分色；②固绿染色液染色：染色4 s，不分色；③醋酸明矾卡红色液染色：染色30 h，分色；④乙醇硼砂卡红染色液染色：染色 22 h，不分色。结果醋酸明矾卡红染色制作的标本囊包轮廓清晰，梭形显著，与周围组织分界明显，囊壁内、外层空隙明显，囊内幼虫清晰。结论醋酸明矾卡红染色制作的标本，染色效果好，易于观察。

关键词：旋毛虫；肌幼虫囊包；染色; 比较

中图分类号：R383.1

文献标识码：A

文章编号：编号： 1672-7606(2015)01-0043-03

收稿日期：2014-10-16

基金项目：河南大学医学院2014年度卓越医师计划项目(2014-17)。

作者简介：马开明(1957-)，男，实验师，山东鄄城人，从事实验技术及实验教学的工作。

通讯作者：*王国英(1962-)，女，山西运城人，硕士，高级实验师，从事寄生虫免疫及寄生虫病流行病学的教学和科研工作。

Abstract:ObjectiveA comparative staining effect of four staining methods for specimen of Trichinella spiralis encapsulated larvae. Methods1) The specimen were stained with Acetate-carmine staining solution for 24 h, and without fading. 2) The specimen were stained with fast green staining solution for 4 s,and without fading. 3) The specimen were stained with acetate alum carmine staining solution for 30 h,and faded. 4) The specimen were stained with alcohol borax carmine staining solution for 22 h,and without fading. ResultsThe results showed that with the acetate alum carmine staining method, specimen had clearly defined capsule edges. It was prominence that third dimension structures of capsulate. The results also showed that the larva, cyst internal layer, cyst external layer and muscle cells were distinguished clearly.Conclusion Capsule showed obvious figure that specimen of acetate alum carmine staining method.

A Comparative Study of Four Staining Methods for specimen of Trichinella spiralis encapsulated larvae

MA Kaiming1, WANG Yaohui1, WU Ming2, FAN Hang2, YAN Yuxin2, CAI Huan2, NIU Lina2, LI Dan2, WANG Guoying1*

(1.LaboratoryofCellularandMolecularImmunology，MedicalCollege，HenanUniversity，Kaifeng,Henan475004，China；2.MedicalCollegeOfHenanUniversity，Kaifeng,Henan475004，China)

Key words: Trichinella spiralis; encapsulated larvae; staining; comparison

旋毛虫病是一种严重的人兽共患寄生虫病，为我国进、出口肉类的首检和必检病种[1]。此病对人类健康和畜牧业生产均构成严重威胁，位列我国三大人兽共患寄生虫病之首(旋毛虫、囊虫及棘球蚴)。在活检的肌肉中查到旋毛虫肌幼虫囊包是确诊旋毛虫病的病原学诊断依据[2]。……