刘小玲，陈晓明,b，阮　晨，许　燕，王　超，廖祥兵，宋　收,罗学刚

(西南科技大学a.生命科学与工程学院，b.核废物与环境安全国防重点学科实验室，中国 绵阳　621010)

微生物培养基中U(Ⅵ)和U(Ⅳ)测定方法的建立及稳定性研究

刘小玲a，陈晓明a,b*，阮晨a，许燕a，王超a，廖祥兵a，宋收a,罗学刚a

(西南科技大学a.生命科学与工程学院，b.核废物与环境安全国防重点学科实验室，中国 绵阳621010)

摘要为了消除微生物培养基对U(Ⅵ)和U(Ⅳ)质量浓度测定的影响，建立微生物培养基中测定U(Ⅵ)和U(Ⅳ)质量浓度ρ的方法，优化了在稀盐酸体系中利用U(Ⅵ)制备U(Ⅳ)的条件：10 mg/L U(Ⅵ)，锌粒用量12 g/L，反应时间180 min.通过测定ρ总U减去ρU(Ⅵ)，计算出ρU(Ⅳ)，建立了同时测量微生物培养基中ρU(Ⅵ)和ρU(Ⅳ)的分光光度法.微生物培养基对U(Ⅵ)和U(Ⅳ)有明显影响:LB和NA培养基对U(Ⅵ)的影响率分别为37.3%和47.9%；单组分中蛋白胨和牛肉膏对U(Ⅵ)的影响率较大，分别达到33.5%和19.7%；而U(Ⅵ)和U(Ⅳ)在TGY培养基中较稳定．采用该方法测量微生物培养基中ρU(Ⅵ)和ρU(Ⅳ)，能消除微生物培养基对U的影响，且具有精密度高、操作简单等优点，能实现生物样品中ρU(Ⅵ)和ρU(Ⅳ)的快速测量．

关键词U(Ⅳ)的制备；分光光度法；微生物培养基；U稳定性

Establishment of Determination Method and Stability of

U(Ⅵ) and U(Ⅳ) in Microbial Medium

LIUXiao-linga,CHENXiao-minga,b*,RUANChena,XUYana,WANGChaoa,

LIAOXiang-bina,SONGShoua,LUOXue-ganga

(a. School of Life Science and Engineering, b. School of National Defense Science and Technology,

Southwest University of Science and Technology, Mianyang 621010, China)

AbstractIn order to eliminate the influence of microbial culture medium to the measurement of U(Ⅵ) and U(Ⅳ) mass concentration and establish detection method of U(Ⅵ) and U(Ⅳ) in microbial medium, the optimal preparation conditions of U(Ⅳ) in dilute hydrochloric acid system were discussed in this study. The optimal preparation conditions of U(Ⅳ) include U(Ⅵ) at a final concentration of 10 mg/L, zinc dosage of 12 g/L, the reaction time of 180 min. U(Ⅳ) by U(Ⅵ) and total U is determined. Spectrophotometry is established to measure the concentration of U(Ⅵ) and U(Ⅳ) in microbial medium. Microbial culture medium has significant effect on the measurement of U(Ⅵ) and U(Ⅳ). The influence rates of LB, NA medium to U(Ⅵ) are 37.3% and 47.9%, respectively. The influence rates of peptone and beef extract in single component to U(Ⅵ) are 33.5% and 19.7%, respectively. U(Ⅵ) and U(Ⅳ) in TGY medium are relatively stable. The proposed spectrophotometry method has high recovery rate, accuracy and precision, simple operation and so on, which could be used to measure the concentrations of U(Ⅵ) and U(Ⅳ) rapidly.

Key wordspreparation of U(Ⅳ); spectrophotometric method; microbial medium; U stability

随着核工业的发展，含U放射性废物不断进入环境中，对人类健康和生态环境造成威胁．地壳表层平均铀含量为3 μg/g[1]．铀矿冶、核电站、核武器实验等含铀废水中，铀的质量浓度约为5 mg/L，远高于国家排放标准(0.05 mg/L)，也远高于世界卫生组织(WHO)规定饮用水标准最高铀质量浓度50 μg/L[2-3]．高浓度铀的放射性和化学毒性影响动物和人体肾脏和骨骼的正常功能[4]，因……