基于多波长覆盖融合指纹图谱的大川芎片主要成分研究

唐爽1，包永睿1,2,3，孟宪生1,2,3，富力4，王帅1,2,3

(1.辽宁中医药大学药学院，辽宁 大连 116600；2.辽宁省组分中药工程技术研究中心，辽宁 大连 116600；

3.辽宁省现代中药研究工程实验室，辽宁 大连 116600；4.大连富生天然药物开发有限公司，辽宁 大连 116600)

摘要：目的建立10批次大川芎片多波长融合高效液相色谱指纹图谱，在此基础上对大川芎片融合指纹图谱共有峰进行初步指认，构建大川芎片的全析指纹图谱为其全面的质量控制提供依据。方法采用高效液相色谱法对大川芎片进行检测，将不同波长下数据进行多波长融合得到10批大川芎片的融合指纹图谱，采用液质联用技术对融合指纹图谱中的共有峰进行初步结构鉴定。结果10批大川芎片融合指纹图谱共获得13个共有峰并与共有模式图相似度均>0.92，其中8个共有峰得到指认。结论本文通过多波长融合技术手段及HPLC-MS分析方法建立大川芎片全析指纹图谱，为大川芎片主要成分的质量控制提供依据，为规范中药制剂及其合理使用奠定基础。

关键词：大川芎片；多波长融合；指纹图谱；液质联用

基金项目：“十二五”“重大新药创制”科技重大专项(No.2013ZX09507005)

作者简介：唐爽，女，研究方向：药物分析，E-mail：707547627@qq.com

通讯作者：孟宪生，男，博士研究生，博士生导师，研究方向：中药组分配伍、代谢组学及药品质量分析，Tel：0411-85890185，E-mail：mxsvvv@126.com

中图分类号：R284.2文献标识码：A

Study on the main components of the Dachuanxiong Tablets based

on multiple wavelength coverage fusion fingerprint

TANGShuang1,BAOYong-rui1,2,3,MENGXian-sheng1,2,3,FULi4,WANGShuai1,2,3

(1.CollegeofPharmacy，LiaoningUniversityofTraditionalChineseMedicine，Dalian116600，China；

2.LiaoningProvinceMulti-ComponentChineseMedicineEngineeringTechnologyResearchCenter，

Dalian116600，China；3.LiaoningProvinceModernTraditionalChineseMedicineResearchand

EngineeringLaboratory，Dalian116600，China;4.DalianFushengNaturalDrug

DevelopmentCo.,Ltd.，Dalian116600，China)

Abstract：ObjectiveTo set up the multi-wavelength fusion HPLC fingerprint of 10 batches of the Dachuanxiong Tablets,common peaks were preliminary identified on that basis,building the total analyzed fingerprint in order to provide a base for the comprehensive quality controlling of the drug.MethodsThe fingerprint under different wavelength for ten batches of Dachuanxiong Tablets were tested by HPLC-DAD and the active components was identified by HPLC-MS.ResultsA HPLC fingerprinting pattern of 10 batches of the Dachuanxiong Tablets was set up based on multi-wavelength fusion,which demarcated 13 common peaks,8 of which were confirmed,the similarity was more than 0.92 between the correlation coefficients of the 10 samples.ConclusionIn this article,with the method of multi-wavelength fusion and HPLC-MS,we established the total analyzed fingerprint of Dachuanxiong Tablets and provided the foundation for the quality controlling of the tablets,which laying a foundation to regulate traditional Chinese medicine preparations and its reasonable application.

Key words:Dachuanxiong Tablets；Multiple wavelength fusion；Fingerprint；HPLC-MS

大川芎片由天麻、川芎两味药材组成，经提取、精制等一系列工艺处理后制成，具有活血化淤、平肝熄风之功效，专门用于治疗各种头痛和眩晕，疗效确切、毒副作用少，已广泛应用于临床。该……