周络通对糖尿病大鼠周围神经形态学改变及神经生长因子含量的影响

姚鸿萍，封卫毅*，魏友霞

(西安交通大学医学部第一附属医院药学部，西安710061)

摘要：目的观察周络通对糖尿病大鼠周围神经形态学改变及神经生长因子(NGF)含量的影响。方法采用链脲佐菌素糖尿病大鼠(STZ-D)模型，灌胃给药8周，锇酸染色观察坐骨神经形态变化，用ELISA法测定糖尿病大鼠血清中的NGF含量，用免疫组化法测定神经组织中NGF含量。结果周络通对糖尿病大鼠坐骨神经形态学改变有明显保护作用；对血清NGF含量减少有明显提高作用；对坐骨神经组织中NGF的含量虽无明显影响，但对坐骨神经轴突内NGF的减少有明显的提高作用。结论周络通对糖尿病大鼠周围神经病变具有保护作用，对血清和坐骨神经轴突内NGF的减少具有明显的提高作用。

关键词：周络通；糖尿病周围神经病变；神经生长因子

doi:10.3969/j.issn.1004-2407.2015.06.017

中图分类号：R965文献标志码：A

作者简介：姚鸿萍，女，硕士研究生，副主任药师

收稿日期：(2015-03-22)

Effect of Zhouluotong on sciatic nerve morphology and nerve growth factor contents in streptozotocin-induced diabetic rats

YAO Hongping，FENG Weiyi*，WEI Youxia (Department of Pharmacy,the First Affiliated Hospital， Health Science Center，

Xi′an Jiaotong University， Xi′an 710061， China )

Abstract:ObjectiveTo investigate the effect of Zhouluotong on sciatic nerve morphology and nerve growth factor contents in streptozotocin-induced diabetic (STZ-D) rats with diabetic peripheral neuropathy (DPN). MethodsDrugs were administrated orally for 8 weeks continuingly after the streptozotocin treatment. Morphological changs in sciatic nerve were observed with histological staining. Nerve growth factor(NGF) contents in plasma and sciatic nerve tissues were determined with ELISA kit and immunohistochemical staining. ResultsMorphological degeneration in sciatic nerve， decrease of nerve growth factor concertration in rat plasma， as well as in nerve axis cylinder were ameliorated by oral administration of Zhouluotong to diabetic rats.Conclusion These observations suggested that Zhouluotong demonstrated curative effect on DPN in STZ-D rats.

Key words: Zhouluotong；diabetic peripheral neuropathy；nerve growth factor

*通信作者:封卫毅，男，博士研究生，主任药师

糖尿病神经病变(diabetic peripheral neuropathy，DPN)是糖尿病三大并发症之一，其发病率和死亡率较高[1]，目前尚没有特效的治疗药物。DPN的发生与体内多种神经体液代谢紊乱有关，其中神经生长因子(nerve growth factor， NGF)含量异常是引起DPN的重要原因之一[2-4]。在周络通临床应用中发现，该药对糖尿病患者的某些外周神经并发症，如:麻木、肢体感觉异常、疼痛等具有明显的改善作用。而我们前期的研究发现[5]，周络通对神经组织多元醇代谢通路的增强具有明显的抑制作用，并可降低神经组织中损伤性多元醇物质的生成。……