原儿茶酸对大鼠心肌缺血/再灌注损伤的保护作用

覃华1，张琰1，杜小燕1，崔颖2，王茹1*

(1.第四军医大学唐都医院药剂科，西安 710038； 2.第四军医大学唐都医院输血科，西安 710038)

摘要：目的研究原儿茶酸对大鼠心肌缺血/再灌注(myocardial ischemia/reperfusion，MI/R)损伤的保护作用。方法采用结扎SD大鼠冠状动脉建立MI/R (30 min/3 h)损伤模型，将动物随机分为假手术组、模型组、原儿茶酸组(低、中、高剂量)、丹参多酚酸盐6组。除模型组于再灌注时给予生理盐水外，原儿茶酸低、中、高剂量组及丹参多酚酸盐组于再灌注即刻给予原儿茶酸(5，10和20 mg·kg-1)及丹参多酚酸盐(20 mg·kg-1)。实验过程中监测心功能，实验末测定心肌梗死面积，并检测血清中肌酸激酶同工酶(CK-MB)、肌钙蛋白(cTnI)水平。结果与模型组相比，原儿茶酸中、高剂量组大鼠心功能得以改善(P<0.05)，心肌梗死面积显著减少(P<0.05)，血清中心肌损伤酶CK-MB、cTnI的水平显著下降(P<0.05)。结论原儿茶酸对大鼠MI/R损伤具有一定的保护作用。

关键词：原儿茶酸；心肌缺血/再灌注；心功能；心肌梗死

doi:10.3969/j.issn.1004-2407.2015.06.016

中图分类号：R965文献标志码：A

基金项目：国家自然科学基金青年科学

作者简介：覃华，女，主管药师

收稿日期：(2015-03-02)

Protective effect of protocatechuic acid on myocardial ischemia/reperfusion injury in rats

QIN Hua1，ZHANG Yan1，DU Xiaoyan1，CUI Ying2，WANG Ru1*(1.Department of Pharmacy，Tangdu Hospital，the Fourth Military Medical University，Xi′an 710038，China；2.Department of Blood Transfusion, Tangdu Hospital, the Fourth Military Medical University, Xi′an 710038，China)

Abstract:ObjectiveTo investigate the protection effect of protocatechuic acid on myocardial ischemia/reperfusion (MI/R) injury in rats. Methods The rats were subjected to MI/R (30 min/3 h) by occluding the left anterior descending artery. The rats randomly received one of the following treatments intravenously and were randomly divided into six groups: sham group，model group，protocatechuic acid group (low，middle and high doses) and salvianolate group. Sham-operated control rats (sham) underwent the same surgical procedures with the exception of left anterior descending coronary artery occlusion. Protocatechuic acid and salvianolate groups were treated with the dose of 5，10 and 20 mg·kg-1 of protocatechuic acid and 20 mg·kg-1 of salvianolate， respectively. The change of cardiac function was monitored by multi-channel physiology recorder. The area of myocardial infarction and the levels of CK-MB，cTnI in serum were determined to evaluate the protection effect of protocatechuic acid. Results Compared with the model group，the cardiac function of MI/R rats was improved(P<0.05)，the infarct area and the levels of creatine kinase-MB (CK-MB) and cardiac troponin (cTnI) in the middle and high dose of protocatechuic acid groups were significantly decreased (P<0.05).ConclusionProtocatechuic acid has excellent cardioprotective effects against MI/R injury in rats.

Key words: protocatechuic acid；myocardial ischemia/reperfusion；cardiac function； myocardial infarction

*通信作者：王茹，女，副主任药师

冠状动脉性心脏病(coronary artery heart disease，CAHD，简称冠心病)对生命和健康的威胁很大，是当今造成人类死亡的头号杀手。临床上冠脉支架、溶栓及搭桥血管重建术等已成为传统的治疗方法，但在心脏血运重建过程中反而出现比心肌缺血更明显、更严重的损伤和功能障碍，称为再灌注损伤。目前，关于心肌缺血/再灌注(myocardial ischemia/reperfusion，MI/R)损伤领域的研究已经受到医药学家的高度关注，探讨如何减轻MI/R损伤是研究的热点[1-2]。中药治疗MI/R损伤疗效确切、前景良好[3]，其中丹参(Salviamiltiorrhizae)心肌保护作用的研究最为引人关注。……