益气扶正复方提取物对小鼠免疫功能及荷瘤生存时间的影响

梁健，梁楚燕，吴亚运，刘华桢，朱冬梅，侯少贞*

(广州中医药大学中药学院，广州 510006)

摘要：目的研究益气扶正复方提取物对小鼠免疫功能及荷瘤生存时间的影响。方法采用小鼠碳廓清法和鸡红细胞所致的血清溶血素抗体生成实验来观察益气扶正复方提取物对免疫的调节作用，通过检测S180腹水瘤小鼠生命延长率实验来测定药物对荷瘤小鼠的影响。结果益气扶正复方提取物低、中、高剂量组的脾脏系数,廓清指数K的值均大于醋酸泼尼松模型组(P<0.05);益气扶正复方提取物低、中、高剂量组及青春宝组的血清溶血素水平的值均大于空白组(P<0.01);益气扶正复方提取物低、中、高剂量组及氟尿嘧啶组的生命延长率均大于空白组(P<0.01)。结论益气扶正复方提取物具有提高机体免疫力和抗肿瘤作用。

关键词：益气扶正；免疫功能；荷瘤小鼠

DOI::10.13463/j.cnki.jlzyy.2015.06.028

中图分类号：R285.5文献标志码： A

文章编号：1003-5699(2015)06-0620-03

基金项目:国家自然科学

作者简介:梁健(1988-)，男，硕士研究生，主要从事中药保健品药物的研发。

收稿日期:(责任编辑：赵玉芝2014-08-24)

*通信作者:侯少贞，电话-(020)39358626，电子信箱-hsz0214@gzuce.edu.cn

Effect of Yiqi righting Compound extract on mice immune system

and the survival time of tumor-bearing mice

LIANG Jian,LIANG Chuyan,WU Yayun,LIU Huazhen,ZHU Dongmei,HOU Shaozhen*

(School of Chinese Pharmaceutical Science,Guangzhou University of Chinese Medicine,Guangzhou,510006,China)

Abstract:ObjectiveTo observe the effect of Yiqi righting Compound extract on mice immune system and the survival time of tumor-bearing mice.MethodsThe immunomodulatory effect of Yiqi righting Compound extract was assessed by determining the capacity of carbon particle clearance in normal mice and generation of hemolysin antibody induced by chicken red blood cell (CRBC).To determine the effect of drugs on tumor-bearing mice by detecting S180 ascites tumor in mice life prolonging rate.ResultsThe atrophy of mouse spleen and clearance index of Yiqi righting compound extract of low,medium and high dose groups have been increased,compared with the Prednisone Acetate model group (P<0.05).The level of serum hemolysin in Yiqi righting compound extract of low,medium and high dose groups and the Qingchunbao Group are higher than the blank group(P<0.01).The prolongation of life span of Yiqi righting compound extract and the fluorouracil group are longer than the blank group (P<0.01).ConclusionYiqi righting compound extract can enhance the immunity function and has antitumor action.

Keywords:Yiqi righting;immune function;tumor-bearing mouse

近年来，中药抗肿瘤和增强机体免疫功能的研究越来越受到人们的重视，中药复方作为中医临床用药的主要形式，体现了中医整体观和辨证论治的特色[1-2]。益气扶正复方主要由黄芪[3]、绞股蓝、丹参、野金柴这4种中药材按照一定比例配伍组成的复方制剂。本实验旨在探讨以补虚药物作为君药组成的益气扶正复方对小鼠免疫功能的影响及其抗肿瘤的作用。

1实验材料

昆明小鼠18～24 g，雌雄各半，广州中医药大学实验动物中心提供，合格证号:SCXK(粤)2008-0020(0065103)。益气扶正复方提取物，广州中医药大学方剂教研室提供。氟脲嘧啶，由广州中医药大学肿瘤教研室惠赠。……