通补消积饮对人肺腺癌A549细胞Cyclin D1蛋白表达的影响

李义君1，全丽娜2，刘建秋3

(1.黑龙江中医药大学附属第二医院，哈尔滨 150001；2.哈尔滨医科大学附属第三医院，哈尔滨 150040；

3.黑龙江中医药大学附属第一医院，哈尔滨 150040)

摘要：目的研究通补消积饮对A549细胞Cyclin D1蛋白表达的影响。方法应用Western-blot法观察通补消积饮对A549细胞Cyclin D1蛋白表达的影响。结果通补消积饮低、中、高剂量组含药血清作用A549细胞48 h后,Cyclin D1蛋白相对表达量分别为(0.739±0.051),(0.520±0.047)，(0.395±0.035)，通补消积饮各剂量组与空白血清组(0.881±0.062)比较，差异有统计学意义(P<0.05，P<0.01)，在一定的时间内表现出明显的量-效关系。结论通补消积饮能明显抑制A549细胞Cyclin D1蛋白的表达。

关键词：通补消积饮；A549；Cyclin D1

DOI::10.13463/j.cnki.jlzyy.2015.06.027

中图分类号：R285.5文献标志码： A

文章编号：1003-5699(2015)06-0617-02

基金项目:黑龙江省教育厅科学技术研究项目(12521509)。

作者简介:李义君(1979-)，男，医学博士，主治医师，主要从事中医药治疗恶性肿瘤的临床与实验研究。

收稿日期:(责任编辑：赵玉芝2014-09-24)

Tongbu Xiaoji decoction on expression of Cyclin D1 in human lung adenocarcinoma cell line A549

LI Yijun1,QUAN Lina2,LIU Jianqiu3

(1.Heilongjiang University Second Affiliated Hospital of Traditional Chinese Medicine,Harbin 150001,China;

2.The Third Affiliated Hospital of Harbin Medical University,Harbin 150040,China;

3.Heilongjiang University First Affiliated Hospital of Traditional Chinese Medicine,Harbin 150040,China)

Abstract:ObjectiveTo investigate the influence of the Tongbu-Xiaoji decoction to the expression of Cyclin D1 of A549 cells.MethodsThe protein expression of Cyclin D1 in A549 cells were evaluated by Western blot.ResultsTongbu-Xiaoji decoction with low、median and high dose medicated serum were used to affected A549 cell.After 48 h,the protein expression of Cyclin D1 of A549 cells significantly decreased and the values were (0.739±0.051),(0.520±0.047) and (0.395±0.035).Compared with that of blank group,Tongbu-Xiaoji decoction with low,median and high dose medicated serum (0.881±0.062) had a significant difference (P<0.05,P<0.01),and the affection shows a dose-dependent manner.ConclusionTongbu Xiaoji decoction inhibited the protein expression of Cyclin D1 in A549 cells significantly.

Keywords:Tongbu Xiaoji decoction;A549;Cyclin D1

中医药在中晚期肺腺癌的治疗上有其独特理念和实际优势[1]。通补消积饮是多年临证中总结的经验方，笔者研究通补消积饮对A549细胞Cyclin D1蛋白表达的影响，总结报道如下。

1实验材料

人肺腺癌A549细胞，Wistar雄性大鼠。二氧化碳孵育箱；医用超净工作台；高速台式离心机；低速离心机；电热恒温水槽；电泳仪；垂直电泳槽及制胶装置；酶标仪。通补消积饮中药复方由黑龙江中医药大学附属二院饮片药局提供。RIPA裂解液；Bradford法蛋白定量试剂盒；辣根过氧化物酶标记山羊抗兔IgG(H+L)；ECL试剂盒；Beta Actin antibody；Cyclin D1 antibody。

2实验方法

2.1含药血清制备Wistar雄性大鼠16只随机分为4组，每组4只。通补消积饮低、中、高剂量组给药剂量分别为28，56，112 g/(kg·d)，分2次灌胃;空白血清组：灌生理盐水，分2次灌胃；……