李涛　苏慧慧　李植良　徐小万　王恒明　李颖　黎振兴

摘 要 组蛋白去乙酰化酶（Histone deacetylases，HDACs）家族基因在植物的生长发育、器官构建及逆境胁迫和激素信号应答中发挥重要作用。利用生物信息学方法对番茄的HDACs家族成员、分布及结构和功能等进行分析。结果表明，番茄HDACs家族包含15个成员，分为3个亚家族。遗传进化分析表明，番茄HDACs家族成员与拟南芥HDACs家族具有相似分类。利用实时荧光定量PCR对番茄HDACs家族基因的组织表达分析表明，HDACs具有组织特异性表达差异，SlHDT1、SlHDT2和SlHDT3在根中表达较高，而SlHDA1、SlHDA3、SlHDA5、SlHDA6在果实发育过程中表达较高；利用RT-PCR对番茄HDACs的胁迫响应分析表明，在盐、SA、ABA、高温和低温胁迫条件下，15个番茄HDACs成员的表达模式不同，其中部分基因的表达水平被显著地诱导增加或者降低，推测这些基因很可能参与了调控番茄逆境胁迫条件下的防御应答反应。结果将为进一步解析番茄HDACs家族基因的功能奠定基础。

关键词 番茄；组蛋白去乙酰化酶；表达分析；氧化胁迫；青枯病

中图分类号 S641.2 文献标识码 A

Abstract Histone acetylation and deacetylation play an important role in the regulation of eukaryotic gene expression， and implicated in plant development， organ formation， stress response， and hormonal signaling. In this study， our analysis revealed the presence of 15 genes encoding HDAC proteins in the tomato genome， the distribution on the chromosome， the structure and function of proteins were analyzed. HDAC proteins could be classified into 3 groups based on phylogenetic analysis. Ten of them belonged to RPD3/HDA1， and other members had similar classification with Arabidopsis thaliana， which was supported with the organization of predicted conserved putative motifs in HDAC proteins. The analysis of the developmental tomato expression profiling data with Q-PC， indicated that SlHDT1， SlHDT2 and SlHDT3 were highly expressed in roots， in addition， the transcripts of SlHDA1， SlHDA3， SlHDA5 and SlHDA6 were accumulated in fruits. Fifteen HDACs genes showed distinct expression patterns in response to stresses of salt， SA， ABA， high temperature and cold， the expressions of several genes were significantly up/down regulated， implying that these members might participate in regulating the defense response against abiotic stresses. The results would provide a very useful reference for the cloning and functional analysis of each member of HDAC gene family in Solanaceae crops.

Key words Tomato； Histone deacetylation； Gene expression； Oxidative stress； Bacterial wilt

doi 10.3969/j.issn.1000-2561.2015.11.012

植物在生长过程中会不断受到来自环境的生物和非生物胁迫，非生物胁迫如干旱、冷害、高盐等，生物胁迫如细菌、真菌、病毒等[1]。植物经胁迫诱导后发生一系列生理生化变化，如多种信号途径的激活，功能性调控蛋白的诱导表达等，来应变和抵抗这些不良环境对其生长发育的影响[2]。组蛋白修饰在基因表达调控方面扮演重要角色，组蛋白通过组蛋白乙酰转移酶（Histone acetyltransferase，HATs）促进基因表达，而组蛋白去乙酰化酶（Histone deacetylase，HDACs）抑制基因表达。……