郑梅竹等

摘要：为了探讨白芍（Radix paeoniae Alba）提取物对MPTP（1-甲基-4-苯基-1，2，3，6-四氢吡啶）致帕金森病小鼠的保护作用，试验采用不同剂量的白芍提取物（0.75、1.50、3.00 g生药/kg体重）灌喂小鼠，每日一次，连续15 d。第八天起所有小鼠每日腹腔注射MPTP 1次共8次，建立小鼠帕金森病（Parkinsons disease，PD）模型，通过检测小鼠的自主活动、爬杆能力、滚轴能力及悬挂能力等行为学表现，同时检测小鼠脑黑质中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）活性及丙二醛（MDA）水平的变化来评价白芍提取物对PD小鼠的影响。结果显示，经MPTP诱导的小鼠，其自主活动次数显著减少、爬杆时间差值显著增加、附着能力评分显著降低；经高剂量白芍提取物预处理后能显著（P<0.05）增加小鼠在测试时间内的自发站立次数，显著（P<0.05）缩短爬杆时间差值，显著（P<0.05）提高附着能力分值，极显著（P<0.01）提高小鼠脑内SOD、GSH-Px水平，MDA水平极显著（P<0.01）。

关键词：白芍（Radix paeoniae Alba）提取物；帕金森病（PD）；小鼠；氧化应激

中图分类号：R742.5；R285.5 文献标识码：A 文章编号：0439-8114（2015）12-2960-04

DOI：10.14088/j.cnki.issn0439-8114.2015.12.039

Neuroprotective Effect and Mechanism of Radix paeoniae alba Extracts Against MPTP-induced Mouse Model of Parkinsons Disease

ZHENG Mei-zhua，FAN Ya-junb，SHI Dong-fanga，ZHANG Yu-chia，LIU Chun-minga，WANG Yue-qia，REN Jun-qia

（Changchun Normal University，a.The Central Laboratory；b.The College of life Science， Changchun 130032， China）

Abstract：For studying the neuroprotective effect of Radix paeoniae Alba extracts against MPTP-induced mouse model of Parkinsons disease （PD） and its mechanism， 60 mice were randomly indiveded to 6 groups， such as nomal control group， model conrol group， positive medicine control group， and three trial groups which treated with Radix Paeoniae Alba extracts of different dots （0.75、1.50、3.00 g/kg，i.g.） for 15 days. At the 8th day， the mice except of the nomal control group were started intraperitoneal injection with MPTP（3.00 g/kg，i.p.） for eight days and the subsequent behavioral testing of mice， such as voluntory movement， rotarod test， pole test and traction test was assessed. SOD、GSH-Px and MDA levels in mouse striatum nigra were measured using analysis Kit. The results showed that the MPTP-induced mice presented typical Parkinsons disease such as voluntory movement significantly decreased， the time difference of climbing pole， significantly increased and the adherence ability assessment significantly decreased， etc. But after pretreated Radix paeoniae Alba extracts， the abnormal behavior including the decrease in spontaneous motor activity and the prolongation in the pole test latent period induced by MPTP were all significantly improved（P<0.05）.In addition， Radix Paeoniae Alba extracts significantly inhibited the oxidative stress level， such as decreased the level of MDA （P<0.01） and increased the antioxidant enzyme GSH-Px（P<0.05） and SOD activity（P<0.05）.

Key words：Radix paeoniae Alba；Parkinsons disease （PD）；mouse；oxidative stress

开发新型、高效、低毒副作用的帕金森病（Parkinsons disease，PD）药物始终是精神药理学的一个重要研究课题，因此从天然药物中寻找高效抗PD制剂成为近几年一个新的研究方向。

白芍（Radix paeoniae Alba）为毛茛科植物芍药（Paeonia lactiflora Pall） 的干燥根，在中国北方地区分布广泛。有养血、益气、止痹、通络的作用，是风湿类疾病、自身免疫病、肝炎及肝硬化治疗中重要的组方之一，受到医药学界的广泛重视。现代药理学表明白芍提取物可改善东莨菪碱所致小鼠的学习记忆障碍，并能增强大鼠的学习能力。研究发现，芍药总苷对大鼠皮层神经细胞缺氧、缺糖、氧自由基及NO神经毒性等损伤有显著保护作用[1]。

PD的发病机制机理有环境和基因两方面的因素，临床研究表明，PD患者处于氧化应激状态，氧化应激反应是PD的发病机制之一[2]，在各种神经变性机制中，氧化应激占据主导地位，并对多巴胺神经元死亡起重要作用[3]。……