徐晓燕等

摘要：为了研究稀土镧（La3+）对铁皮石斛（Dendrobium officinale Kimura et Migo）幼苗生理特性和品质的影响，在温室里用5～120 mg/L的镧溶液分别对铁皮石斛幼苗进行了喷施试验。结果显示，随着镧溶液浓度的升高，幼苗叶片和茎秆中叶绿素、可溶性蛋白、脯氨酸、多糖、石斛碱的含量呈先增加、后降低的变化趋势，而丙二醛的含量先降低、后增加。综合而言，用20 mg/L镧溶液浓度喷施铁皮石斛幼苗为最佳处理浓度，此时叶片中叶绿素的含量比空白对照增加54.37%、可溶性蛋白含量增加53.64%、脯氨酸含量增加99.20 %、丙二醛含量降低44.71%、多糖含量增加56.60%、石斛碱含量增加14.06%；茎秆中叶绿素的含量比空白对照增加17.48%、可溶性蛋白含量增加56.57%、脯氨酸含量增加253.64%、丙二醛含量降低74.91%、多糖含量增加88.73%、石斛碱含量增加24.54%。表明20 mg/L的镧溶液浓度处理能有效的促进铁皮石斛的光合作用、增强抗逆性和提高品质。

关键词：镧；铁皮石斛（Dendrobium officinale Kimura et Migo）；生理指标；品质

中图分类号：O614..33+1；S567.23+9；Q945 文献标识码：A 文章编号：0439-8114（2015）12-2935-04

DOI：10.14088/j.cnki.issn0439-8114.2015.12.032

Effect of La3+ on Physiological Characteristics and Quality of

Dendrobium officinale Seedlings

XU Xiao-yan， TANG Di， CAI Jing-wen， ZHU Hui-fei， SHI Jun

（Jiangsu Polytechnic College of Agriculture & Forestry， Jurong 212400， Jiangsu， China）

Abstract： In order to study the effect of different concentration of Lanthanum （La3+） on some physiological characteristics and quality of Dendrobium officinale Kimura et Migo seedlings， the seedlings was sprayed by 5 to 120 mg/L La3+ in the greenhouse. The results showed that the content of chlorophyll， proline， soluble protein， polysaccharide and dendrobine in leaves and stems increased and then decreased with the increasing of La3+ concentration； while the content of malondialdehyde decreased before it increased. Combined with all the indexes， 20 mg/L La3+ solution was the best treatment concentration as the content of chlorophyll increased by 54.37%； soluble protein increased by 53.64%， proline increased by 99.20%， malondialdehyde decreased by 44.71%， polysaccharide increased by 56.60% and dendrobine increased by 14.06% in leaves compared with the control. The content of chlorophyll increased by 17.48%， soluble protein increased by 56.57%， proline increased by 253.64%， malondialdehyde decreased by 74.91%， polysaccharide increased by 88.73% and dendrobine increased by 24.54% in stems， respectively. The concentration of 20 mg/L La3+ could effectively promote the photosynthesis， enhance the resistance and improve quality of D. officinale seedlings.

Key words： Lanthanum； Dendrobium officinale Kimura et Migo； physiological characteristics； quality

铁皮石斛（Dendrobium officinale Kimura et Migo）是多年生草本植物，属于国家二级保护植物，为《中华人民共和国药典》中记载的一种名贵中药材，具有益胃生津、滋阴清热等功效[1]。铁皮石斛的主要成分为多糖和生物碱，以及微量的茋类、糖苷类等化合物[2]。现代药理学研究表明，铁皮石斛具有增强免疫力、抗氧化、抗肿瘤、降血糖等功效[3，4]，是部分中药制剂、中成药、保健品的必需原料。近年来，由于过度采挖和生态环境的破坏，致使野生资源濒临枯竭；加上铁皮石斛的生长对环境条件要求苛刻，所以人工栽培的铁皮石斛生长较缓慢，产量也低。……