游泳+杜莹莹+曹媛+李真珍+张胜军

[摘要] 目的 分析基质金属蛋白酶2（MMP-2）和MMP-9及其抑制因子2（TIMP-2）和TIMP-1联合检测对宫颈癌浸润转移潜能的预测价值。 方法 选取经病理证实的宫颈浸润癌患者112例（ICC组）、宫颈上皮内瘤样病变患者56例（CIN组）；同时，选取正常宫颈组织标本30例为对照组。采用SABC法行免疫组织化学检测MMP-2、MMP-9、TIMP-2和TIMP-1的表达情况。比较3组相关因子阳性表达率的差异，并分析ICC组各因子阳性表达率与临床病理参数的关系。 结果 ICC组MMP-2和MMP-9的阳性表达率显著高于CIN组和对照组，TIMP-2和TIMP-1的阳性表达率显著低于CIN组和对照组（P<0.05）。ICC组中，MMP-2、MMP-9、TIMP-2和TIMP-1的阳性表达率与国际妇产科联盟（FIGO）分期、组织学分级、分化程度、脉管浸润和淋巴结转移有关（P<0.05）。 结论 MMP-2、MMP-9及其抑制因子TIMP-2、TIMP-1可能与宫颈癌的发生、发展有关，其可作为评估宫颈癌侵袭转移潜能的重要指标。

[关键词] 基质金属蛋白酶；基质金属蛋白酶组织抑制物；宫颈癌

[中图分类号] R737.33[文献标识码] A[文章编号] 1674-4721（2014）05（c）-0104-04

Evaluated value of invasion and metastasis of combined examination of MMP-2,MMP-9 and TIMP-2,TIMP-1 in cervical cancer

YOU Yong1 DU Ying-ying1 CAO Yuan1 LI Zhen-zhen2 ZHANG Sheng-jun2

1.Department of Gynaecology and Obstetrics,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China;2.The Open Key Laboratory of Clinical Medical Experimental Research of Universities in Henan Province,Zhengzhou 450052,China

[Abstract] Objective To investigate the evaluated value of invasion and metastasis of combined examination of matrix metalloproteinase (MMP-2,MMP-9) and tissue inhibitor of matrix metalloproteinase (TIMP-2,TIMP-1) in cervical cancer. Methods 112 cases of patients with early invasive cervical cancer (ICC) and 56 cases of patients with cervical intraepithelial neoplasm (CIN) were selected,and 30 cases of normal cervical epithelium (NCE) were selected as control group.The expression of MMP-2,MMP-9,TIMP-2 and TIMP-1 were examined by SABC method of immunohistochemistry.Expression rate of every factor in different groups and the factors related to clinical pathology of ICC group were analyzed. Rusults Compared with control group and CIN group,expression rate of MMP-2,MMP-9 in ICC group were higher,TIMP-2 and TIMP-1 in ICC group were lower (P<0.05).In ICC group,expression rate of MMP-2,MMP-9,TIMP-2,TIMP-1 were correlated with FIGO clinical stage,histological grading,differentiated degree,intravascular infiltration and lymph node metastasis (P<0.05). Conclusion MMP-2,MMP-9 and TIMP-2,TIMP-1 may play an important role in ivasion and metastasis of cervical cancer.The expression can be used to estimate the metastasis potentiality.

[Key words] Matrix metalloproteinase;Tissue inhibitor of matrix metalloproteinase;Cervical cancer

宫颈癌是女性常见的恶性肿瘤之一，位居生殖器官肿瘤死因的首位。虽然以手术、化疗和放疗为主的综合治疗的疗效有所提高，但预后仍不甚理想[1]。肿瘤的侵袭和转移是导致宫颈癌患者预后不佳的主要原因[2]，如何有效预测宫颈癌的侵袭转移潜能是当前亟待解决的课题。基质金属蛋白酶（matrix metalloproteinase，MMPs）在肿瘤的发生、侵袭转移中发挥重要作用，其抑制物（tissue inhibitor of matrix metalloproteinase，TIMPs）可特异性抑制MMPs的活性[3]。目前，已有少量文献对MMPs/TIMPs在宫颈癌中的表达情况进行报道[4]，但有关联合检测MMPs和TIMPs预测宫颈癌浸润转移潜能的研究较少。……