黄伟强

[摘要] 目的 探讨凋亡抑制蛋白Livin在结肠癌组织中的表达及与细胞增殖的关系。 方法 选取结肠癌患者术后病理标本70例，采用S-P免疫组化法检测Livin基因的表达，并探讨其与患者性别、年龄、肿瘤大小、组织分化程度、浸润深度、淋巴结转移和远处转移等生物学行为及细胞增殖的关系。另取30例癌旁正常的结肠组织作对照。结果 结肠癌组织中Livin基因的阳性率明显高于癌旁正常结肠组织（χ2=12.19，P <0.01）。Livin基因在结肠癌组织中表达水平与性别、年龄、肿瘤大小及组织分化程度无关（P >0.05），随原发病灶的浸润深度、淋巴结转移和远处转移呈明显上升趋势（P <0.01或P <0.05）。Livin基因表达阴性的结肠癌组织中PCNA的细胞增殖指数明显低于Livin基因表达阳性的组织（P <0.05）。 结论 Livin基因不仅参与结肠癌的发病进展过程，而且还参与了结肠癌的局部侵袭和转移的病理过程。Livin不仅参与结肠癌细胞凋亡的调控，还促进了细胞的增殖过程，可作为大肠癌临床诊断、转移和放化疗疗效及预后评估的敏感指标。

[关键词] 结肠癌；免疫组化；Livin基因；细胞增殖

[中图分类号] R735.35 [文献标识码] B [文章编号] 1673-9701（2014）12-0085-03

[Abstract] Objective To discuss expression of inhibitor of apoptosis protein Livin in colon cancer tissue and its relationship with cell multiplication. Methods A total of 70 cases of postoperative pathological specimen of patients with colon cancer tissue were selected， and the expression of Livin genes was detected by S-P immunohistochemistry， whose relationship with gender， age， size of tumor， histological grade， inasie depth， lymphatic metastasis， distant metastasis and other biological behaviour and cell multiplication of patients were discussed. 30 cases of normal colonic tissue near tumor tissues were additionally selected as the research comparison. Results The positive expression rate of Livin genes in colon cancer tissue was much higher than that in normal colon tissue（χ2=12.19， P <0.01）. The expression level of Livin genes in colon cancer tissue has no relationship with gender， age， size of tumor and histological grade（P >0.05）. As the development of inasie depth of primary lesion， lymphatic metastasis and distant metastasis were showed the rising trend （P <0.01 or P <0.05）. The PCNA cell proliferation index in colon cancer tissue of Livin genes negative expression was much lower than that in Livin genes positive expression（P <0.05）. Conclusion Livin genes participate in not only the disease progression of colon cancer tissue， but also pathologic process of local invasion and transformation. Livin genes participate in not only the apoptosis control of colon cancer tissue， but also the boosting of processing of cell multiplication， which can be the clinical diagnosis， transformation， curative effect of radiotherapy and chemotherapy and reliable index of prognosis evaluation.

[Key words] Colon cancer tissue； Immunohistochemical； Livin gene； Cell multiplication

肿瘤的发生发展过程不仅与细胞过度增生有关，而且与细胞的凋亡受抑制密切相关，细胞凋亡途径的调节障碍及凋亡相关基因表达异常，均可导致肿瘤发生和发展。Livin基因属凋亡抑制蛋白（IAPs）家族的抑制细胞凋亡新成员，选择性表达于人的胚胎和大部分肿瘤组织，而在大部分正常人的终末分化组织中无明显表达或低表达[1，2]。有关Livin基因在结肠癌组织中的表达及临床意义近年来逐渐引起临床重视，但其与患者生物学行为的关系目前国内外报道不一致[3，4]。本研究观察了Livin基因在结肠癌组织中的表达，并初步探讨其与患者生物学行为及细胞增殖的关系。现报道如下。endprint

1 资料与方法

1.1 一般资料

本研究共选取了结肠癌患者术后病理标本70份。所有患者具有完整临床资料，术前均未进行任何形式的辅助化疗、放疗和免疫治疗。……