黄坚　王珏　林珍云　商宏恺　马林　卓广超　冯国芳　张治芬

Toll样受体2、3、4、9在胎盘组织中的表达及其与自发性早产的关系

黄坚王珏林珍云商宏恺马林卓广超冯国芳张治芬

【摘要 】目的探讨Toll样受体（TLRs）2、3、4、9在不同孕期胎盘组织中的表达及其与自发性早产的关系。 方法选择自发性早产患者的胎盘样本38份作为早产组（其中孕28-34周和孕35～36周各19份）。选择同期正常妊娠自然分娩产妇的胎盘样本38份（孕37-40周）作为对照组。采用real-time PCR法检测两组胎盘中TLR 2、3、4、9 mRNA的表达，并用Western blot法检测胎盘中TLRs 2、3、4、9蛋白的表达，对结果进行统计学分析。 结果 （1）两组孕产妇胎盘组织中均可检测到TLRs 2、3、4、9的表达。（2）早产组胎盘组织TLR 2、3、9 mRNA的表达均高于对照组，两者间差异有统计学意义（均P＜0.05）；TLR4 mRNA的表达两组间无统计学差异（P＞0.05）。（3）早产组TLR 2、3、4、9的蛋白表达均高于对照组，两者差异均有统计学意义（均P＜0.05）。（4）早产组中，28～34孕周组和35～36孕周组胎盘中TLRs mRNA表达量均无统计学差异（均P＞0.05）。 结论早产患者胎盘组织中TLRs过度表达，提示TLRs介导的母胎界面的免疫反应可能是启动自发性早产的重要因素。

【关键词 】Toll样受体早产免疫反应胎盘

【 Abstract】ObjectiveTo investigate the expression of Toll-like receptors(TLRs)in placenta and its relationship with spontaneous preterm labor.MethodsThirty eight placental samples were collected from patients with spontaneous preterm labor(preterm group),including 19 of 28～34 week gestation,and 19 of 35～36 week gestation.Thirty eight placental samples from normal pregnancy and natural delivery(37～40 week)served as control group.The mRNA expressions of TLR 2,3,4,and 9 were detected by real-time quantitative polymerase chain reaction(RT-PCR);and the protein expression of TLR2,3,4,and 9 were evaluated by Western blot.ResultsThe mRNA expressions of TLR2,3,9 in preterm group were significantly higher than those in control group(P＜0.05),but not for TLR4(P＞0.05).The protein expressions of TLR2,3,4 and 9 also showed higher in preterm group than those in control group(P＜0.05).ConclusionThe over-expressed TLRs in preterm placental tissue suggests that the immune response mediated by TLRs in the maternal-fetal interface may be important factors to initiate spontaneous preterm labor.

【 Key words】 Toll-like receptorsPreterm birthImmune responsePlacenta

早产的发病机制至今尚未完全阐明。近年来研究表明，自发性早产的发生可能是促炎症细胞因子介导母胎界面炎症反应，使胎儿失去免疫保护并被母体内源性免疫排斥的结果。胎儿和母体对各种因素刺激产生的免疫内分泌反应可能是关键原因。目前认为，Toll样受体（Toll-like receptors，TLRs）在内源性免疫系统中起着重要作用，与自发性早产密切相关[1]。

TLRs是天然免疫中重要的模式识别受体，通过对病原微生物的病原相关分子模式的识别激活先天性免疫应答,在机体抵挡病原体感染中发挥重要作用。……