任玲君，方丽娟，童向民

(1.萧山区第四人民医院，浙江 萧山 311225；2. 浙江大学医学院附属第一医院，浙江 杭州 310003)

·基础与临床研究·

2种不同分析仪测定全血CRP结果比较

任玲君1，方丽娟1，童向民2

(1.萧山区第四人民医院，浙江 萧山 311225；2. 浙江大学医学院附属第一医院，浙江 杭州 310003)

目的：比较挪威NycoCard Reader Ⅱ金标检测仪和国赛特定蛋白分析仪2种仪器检测全血C反应蛋白(CRP)的性能及相关性。方法：对2种仪器测定全血CRP的性能进行回归相关性分析。结果高、中、低3个标本的均值和变异系数，挪威NycoCard Reader Ⅱ金标检测仪分别为142.2mg/L CV%=3.89；28.5 mg/L CV%=1.5；12.3mg/L CV%=1.63。而国赛特定蛋白分析分别为106.94 mg/L CV%=6.14；69.32mg/L CV%=4.82；13.6 mg/L CV%=4.49。2种仪器相关实验回归方程为：Y(国)=0.591X(Ny)+26.32；r=0.974。结论2种仪器测定的线性范围有较大差别，但是还存在一定相关性。挪威NycoCard Reader Ⅱ金标检测仪比国赛特定蛋白分析仪在精密度和线性方面更好。

C反应蛋白；精密度，线性

Abstract: [Objective] To compare the two equipments' performance and relevance of Norwegian NycoCard Reader Ⅱ gold detector and Guo-Sai specific protein analyzer in testing C-reactive protein (CRP). [Method] CRP concentration in whole blood was detected by these two equipments. Regression correlation analysis was carried out to compare the results. [Result] The mean and coefficient of variation of high, medium and low concentration of CRP detected by Norway NycoCard Reader Ⅱ gold detectors were 142.2mg / L CV% = 3.89, 28.5 mg / L CV% = 1.5 and 12.3 mg / L CV% = 1.63, respectively. The mean and coefficient of variation of high, medium and low concentration of CRP detected by the Guo-Sai analysis of race-specific proteins were 106.94 mg / L CV% = 6.14, 69.32 mg / L CV% = 4.82 and 13.6 mg / L CV% = 4.49, respectively. Related experimental regression equation was Y (Guo) = 0.591X (Ny) +26.32 r = 0.674. [Conclusion] The linear range of the two devices is quite different, but there is still some relevance. Norway NycoCard Reader Ⅱ gold detector has better precision and linearity than the Guo-Sai specific protein analyzer.

Keywords:C-reactive protein；precision；linearity

C反应蛋白(C-Reactive Protein ；CRP)是一种急性时相反应蛋白，不仅是炎症反应的重要监测指标，而且在诊断糖尿病、冠心病以及移植排斥方面也有较大作用[1-2]。全血快速检测法测定CRP是临床最常见的检测方法。目前应用较多的就是金标定量法、免疫比浊法和胶乳凝集法等，它们都具有方便快捷的特点，但用这些方法学所组成的检测系统，其检测结果是否具有可比性还了解不多。为此，我们对挪威NycoCard Reader Ⅱ金标检测仪和国赛特定蛋白分析仪测定全血CRP进行了比较。

1 材料和方法

1．1 仪器

挪威NycoCard Reader Ⅱ金标检测仪和国赛特定蛋白分析仪。

1．2 试剂和方法

2种仪器均用各自配套专用试剂，并根据仪器和试剂说明书，严格按照操作规程测定。……