[摘要] 目的" 探讨长链非编码RNA MCTP1-AS1对宫颈癌细胞增殖和侵袭的影响及相关机制。方法 采用癌症基因组图谱数据库分析MCTP1-AS1在宫颈癌组织和正常宫颈组织中的表达，分析MCTP1-AS1表达水平与宫颈癌患者病理分期的相关性。检测宫颈癌细胞系HCC1106、HCC94、SiHa、Hela、C33A和宫颈正常上皮细胞H8中MCTP1-AS1的表达。将pcDNA-Ctrl质粒（Ctrl组）和pcDNA-MCTP1-AS1质粒（MCTP1-AS1组）分别转染Hela细胞。检测Hela细胞的增殖和侵袭能力，检测Hela细胞中增殖蛋白CDK2、Cyclin A及侵袭蛋白N-cadherin、ZEB1表达，验证MCTP1-AS1与miR-10a-5p的靶向关系。检测Hela细胞中miR-10a-5p的表达。结果 与正常宫颈组织比较，宫颈癌组织中MCTP1-AS1表达明显降低（Plt;0.01）。MCTP1-AS1表达水平与宫颈癌患者的病理分期呈负相关（Plt;0.01）。与H8细胞比较，宫颈癌细胞系HCC1106、HCC94、SiHa、Hela、C33A中MCTP1-AS1表达均明显降低，pcDNA-MCTP1-AS1质粒可显著增加Hela细胞中MCTP1-AS1表达，过表达MCTP1-AS1能显著降低Hela细胞的增殖和侵袭能力（Plt;0.01）。与Ctrl组比较，过表达MCTP1-AS1可显著降低Hela细胞中增殖蛋白CDK2、Cyclin A及侵袭蛋白N-cadherin、ZEB1。MCTP1-AS1可直接结合miR-10a-5p（Plt;0.01）。与Ctrl组比较，MCTP1-AS1组Hela细胞中miR-10a-5p表达明显降低（Plt;0.01）。结论 宫颈癌组织和细胞中的MCTP1-AS1低表达，且MCTP1-AS1表达与宫颈癌患者的病理分期呈负相关，MCTP1-AS1通过靶向miR-10a-5p抑制宫颈癌细胞的增殖和侵袭能力。

[关键词] 宫颈肿瘤；MCTP1-AS1；miR-10a-5p；细胞增殖；细胞侵袭

[中图分类号] R737.33" """"[文献标识码] A """""[DOI] 10.3969/j.issn.1673-9701.2025.20.001

Expression of lncRNA MCTP1-AS1 in cervical cancer tissues and its regulatory mechanism on proliferation and invasion of cervical cancer cells

CAO Linyan¹， LIU Fang¹， HAN Jing¹， XIA Xinyi¹， GAO Jie²， ZHOU Jiayan¹

1.Department of Gynecology， Jiaxing Second Hospital （Second Affiliated Hospital of Jiaxing University）， Jiaxing 314000， Zhejiang， China; 2.Department of Gynecology， The Second Hospital of Shandong University， Jinan 250033， Shandong， China

[Abstract] Objective To investigate the effect of long noncoding RNA MCTP1-AS1 on the proliferation and invasion of cervical cancer cells and its related mechanisms. Methods The Cancer Genome Atlas database was used to analyze the expression of MCTP1-AS1 in cervical cancer tissues and normal cervical tissues， and the correlation between the expression level of MCTP1-AS1 and the pathological stage of cervical cancer patients was analyzed. The expression of MCTP1-AS1 in cervical cancer cell lines HCC1106， HCC94， SiHa， Hela， C33A and normal cervical epithelial cells H8 was detected. Hela cells were transfected with pcDNA-Ctrl plasmid （Ctrl group） and pcDNA-MCTP1-AS1 plasmid （MCTP1-AS1 group）， respectively. the proliferation and invasion ability of Hela cells were detected， respectively. the expression of proliferation proteins CDK2 and Cyclin A and invasion proteins N-cadherin and ZEB1 in Hela cells were detected， the targeting relationship between MCTP1-AS1 and miR-10a-5p were verified. The expression of miR-10a-5p in Hela cells was detected. Results Compared with normal cervical tissue， the expression of MCTP1-AS1 in cervical cancer tissue was significantly decreased （Plt;0.01）. The expression level of MCTP1-AS1 was negatively correlated with the pathological stage of cervical cancer patients （Plt;0.01）. Compared with H8 cells， the expression of MCTP1-AS1 in cervical cancer cell lines HCC1106， HCC94， SiHa， Hela， and C33A were significantly decreased （Plt;0.01）. Compared to Ctrl group， overexpression of MCTP1-AS1 significantly reduced the levels of proliferative proteins CDK2 and Cyclin A， as well as invasive proteins N-cadherin and ZEB1 in Hela cells. MCTP1-AS1 directly binds to miR-10a-5p （Plt;0.01）. Compared to Ctrl group， MCTP1-AS1 group showed a significant decrease in miR-10a-5p expression in Hela cells （Plt;0.01）. Conclusion MCTP1-AS1 expression is downregulated in cervical cancer tissues and cells， and MCTP1-AS1 expression is negatively correlated with the pathological stage of cervical cancer patients. MCTP1-AS1 inhibits the proliferation and invasion of cervical cancer cells by targeting miR-10a-5p.

[Key words] Cervical tumor; MCTP1-AS1; miR-10a-5p; Cell proliferation; Cell migration

宫颈癌是女性生殖系统最常见恶性肿瘤之一。目前，宫颈癌的发病机制并未完全明确^[1]。研究证实长链非编码RNA（long chain non-coding RNA，lncRNA）在宫颈癌的发生和发展中具有重要调控作用^[2]。越来越多的lncRNA被证明在宫颈癌组织中高表达或低表达，且其表达与宫颈癌细胞的转移潜能（如淋巴结转移或远处转移）有关^[3-5]。LncRNA表达改变与宫颈癌的患病风险相关，不同病理分型的宫颈癌组织中lncRNA表达不同^[6-8]。……