中图分类号：S54 文献标识码：A 文章编号：1007-0435（2025）07-2099-15

doi：10.11733/j.issn.1007-0435.2025.07.006

引用格式：，等.象草PHR家族基因的鉴定及表达分析[J].草地学报，2025，33（7）：2099—2113 FANG Ting，LUO Jia-jia， ZHANG Yu-ge，et al. Identification and Expression Analysis of the PHR GeneFamily in Pennisetum Purpureum[J].Acta Agrestia Sinica，2025，33（7） ：2099-2113

Identification and Expression Analysis of the PHR Gene Family in Pennisetum Purpureum

FANG Tingl#，LUO Jia-jia ^1，2#_. ， ZHANG Yu-ge1，MAO Yu-ye1，GUO Yu-tong1， DONG Rong-shu²， CAI Ze-ping1* （1.SchooloficalAgiclureandFestryanUvesityo，inrovie28a;oicalCoetic Resources Institute，Chinese AcademyofTropical Agriculture Sciences，KeyLaboratoryofCropGeneResourcesand Germplasm EnhancementinSouthernChina，MinstryofAgricultureandRuralAfairs，Haikou，HainanProvince57o，China）

Abstract：The phosphate starvation response （PHR） factor plays a pivotal role in regulating phosphorus homeo stasis in plants，yet the PHRs in Pennisetum purpureum （elephant grass）remain unreported. This study analyzed the efects of low phosphorus （P）stress on the growth of elephant grass，identified the members of PHR family in elephant grass，and examined their transcription expression levels.Furthermore，the two key genes P/PHR/13 were cloned，and their expression patterns and subcellular localization were analyzed. The results demonstrated that low P stress significantly inhibited the growth of elephant grassA total of 42 PpPHRs were identified from the elephant grassgenome，with colliear gene pairs originating from whole-genome duplication or segmental duplication events. Transcriptome showed that 1 5PρPHRs were significantly upregulated by low P stress in elephant grass leaves（and/or roots），with P/PHR/13 exhibiting relatively high expression levels. Additionally， tissue-specific expression analysis revealed that P/PHR/13 had the highest expression levels in roots and stems，respectively. Quantitative PCR confirmed that low P treatment enhanced the expression of

P/PHR/1/3 in both roots and leaves. Subcellular localization indicated that both P/PPHRδ andPpPHR13pro teins were localized in the nucleus. This study provides candidate gene resources for elucidating the mechanisms underlying elephant grass's response to phosphorus deficiency.

Key Words：Pennisetum purpureum;Low phosphorus stress ;Phosphate starvation response factor;Gene expres sion;Subcellular localization

磷（Phosphorus，P）是植物生长所必需的大量营养元素之一，是生物大分子（如核酸、磷脂和三磷酸腺苷ATP等）的基本结构组分，主要通过磷酸化和去磷酸化作用参与光合作用、能量代谢和细胞信号转导等多种重要生物过程[1]。虽然土壤中磷含量丰富，但大部分以有机磷和无机难溶性磷酸盐的形式存在，而植物能直接吸收利用的无机磷酸盐（ H₂PO^4- ，Phosphate inorganic，Pi）含量不足 1% 。为缓解磷有效性低的问题，农民主要通过大量施用磷肥以期提高作物产量，但磷在土中容易被固定为不可直接吸收的磷复合物形式（如在酸性土壤中容易被固定为铁磷和铝磷，在碱性土壤中容易被固定为钙磷），且大部分容易随土壤淋溶进入江河，以引起水体富营养化的环境污染，实质上作物的磷利用效率普遍偏低[2]。全世界范围内，约 40%～60% 可耕地的作物都受到低磷有效性的限制[3]。因此，有效提高作物的磷利用效率（Phosphorusutilizationeffi-ciency，PUE）已成为农业生产中亟待解决的重要问题，而阐明植物对低磷的适应机制是前提。在有效磷（Pi）不足的环境中，植物已经进化出一系列复杂而精细的分子调控网络，以加快外部磷的摄取（磷的吸收）和增强内部磷的循环（磷的利用），其中PHR（Phosphate starvationresponse）蛋白被一致认为是磷信号通路的核心调控因子，参与调控磷稳态[4]。

拟南芥（Arabidopsisthaliana）AtPHR1是第一个被鉴定出的参与磷代谢的重要调节因子，研究发现AtPHR1超量表达能提高拟南芥叶片中的磷含量[5]。……