doi：10.11733/j.issn.1007-0435.2025.07.020

引用格式：，等.23份紫花苜蓿材料萌发期的耐盐性综合评价[J].草地学报，2025，33（7）：2249—2261 ZENG Yi，YUAN Jia-miao，YANG Jing，et al.Comprehensive Evaluation of Salt Toleranceof 23 Alfalfa Materials during Seeds Germination Stage[J].Acta Agrestia Sinica，2O25，33（7） ：2249-2261

中图分类号：S332.6 文献标识码：A 文章编号：1007-0435（2025）07-2249-13

Comprehensive Evaluation of Salt Tolerance of 23 Alfalfa Materials during Seeds Germination Stage

ZENG Yi，YUAN Jia-miao，YANG Jing，WANG Yu-xiang （KeyLaboratoadoudostucatDsetgieboat ResourcesandEcoogofXinjangCollgeofrassandIdustryXinjangAgriculturalUnversity，Urumi，Xijang83oina）

Abstract：To explore salt tolerance of alfalfa during seeds germination period，18 alfalfa（Medicago satiua L.） germplasm resources and 5cultivated varieties were used to study the effects of NaClsolution with diferent conductivity（Electrical，EC）values on seeds germination rate，germination potentialand germination index during sed germination period.The salt resistance of the test materials was evaluated by principal component analyses and membership function.The results showed that the germination response ofdiferent alfalfa materials showed differenttrends，and the performance of the indexeswas quite different.The23 materials were evaluvated comprehensively by using salt tolerance coeficient and comprehensive evaluation D value and systematic cluster analysis.Based on D value，the 23 materials could be divided into 5categories：ClassIwith 4 materials with D value of O.76O，category 8 materials with D value of 0.67O，category 6 materials with D value of 0.536，category 4 materials with a D value of O.43O.Class Vwith only1 material with D value of O.241.Among the 23 materials tested，A1Z 003，A1Z O16，A1Z 005，etc.；A1Z 001，A1Z 006，A1Z 015，etc. Through principal component and correlation analysis，germination rate，germination potential and germination index can be selected as the main indicators of salt tolerance during seeds germination period.This study can provide a theo retical reference for the breeding of saline-alkali tolerant alfalfa varieties.

Keywords：Germination;Salt stress;Conductivity;Membership function

土壤盐渍化是危害生态环境发展的全球性问题，同时也是中国生态环境与农业生产长期平稳发展需要处理的主要问题，严重影响区域农业的可持续发展[1-3]。随着全球人口的不断增长和城市化步伐的加速，对蔬菜、水果以及粮食的需求日益增加，这无疑增加了对耕地资源的依赖和压力。生态环境面临的挑战也日益严峻，特别是土壤盐碱化的问题正在逐渐加剧[4-5]。新疆作为中国盐碱地较为集中的区域之一，其土壤盐碱化问题日益严重6，且受到了多种因素的影响，如地形、气候、水土特性以及人类活动等。盐胁迫是一种广泛存在的非生物胁迫，它对植物的生长和发育有着显著的负面影响。这种胁迫是通过渗透和离子毒害对植物进行生长抑制、发育障碍、种子萌发受阻等迫害[。因此，耐盐植物的深入挖掘及耐盐品种的选育和评价，是未来盐碱土地农业规模化和产业形式生产的重要基石。

紫花苜蓿（MedicagosatiuaL.）是多年生豆科牧草，因其蛋白含量高、抗逆性强、分布广泛等特点被誉为\"牧草之王\"[8-9]，因具有产量高、营养价值丰富、适口性好是发展畜牧养殖业的优质饲料，因此在畜牧业发展中发挥重要作用[10]。紫花苜蓿不仅在低温[11]、干旱[12]等胁迫下均具有很强的抗逆性，还具有一定的耐盐碱性，故其在世界范围内广泛分布。在我国，紫花苜蓿主要分布在华北、东北及西北等地区[13]，这些地区的土壤盐碱化问题较为严重，通过种植紫花苜蓿，不仅可以提供高质量的饲料资源，还可以有效改善土壤环境，促进农业的可持续发展。……