晏和国 侯建婷 尹朝兰 杨博 陈婉丽 李军

【摘要】目的：糖周靈胶囊对STZ诱导糖尿病大鼠下肢神经机械痛阈的影响。方法：将60只雄性SD大鼠适应性饲养1周后，随机分为正常对照组和造模组，其中正常组（10只），造模组分为糖尿病组、糖周灵小剂量组、中剂量组、高剂量组、维生素c组各10只;造模成功后，糖周灵小剂量组以糖尿病0.44g/（kg·d）给药;中剂量组按0.88g/（kg·d）给药;大剂量组按1.75g/（kg·d）给药，维生素C组按0.05g/（kg·d）给药，糖尿病组及对照组给予等量蒸馏水，给药时间为16周，16周后用Von Frey测量仪测定机械痛阈值，观察糖周灵胶囊对糖尿病大鼠下肢神经机械痛阈的影响。结果：正常对照组与维生素C组存在明显差异（P<0.05）;正常对照组、模型组与小剂量组存在明显差异（P<0.01）;模型组与小剂量组、中剂量组、大剂量组存在显著差异（P<0.01），与维C组相比，糖周灵小、中、大剂量组存在明显差异（P<0.05），与糖周灵大剂量相比，糖周灵小、中剂量无明显差异（P<0.05）。结论：糖尿病模型组机械痛阈值最低，糖周灵糖周灵胶囊一定程度上可以提高糖尿病大鼠机械痛阈值，改善糖尿病神经病变神经功能，对糖尿病周围神经病变具有一定保护作用。

【关键词】糖周灵胶囊;糖尿病;机械痛阈值

【中图分类号】R285.5【文献标志码】 A【文章编号】1007-8517（2021）18-0012-04

Effects of  Tangzhouling Capsule on Stz-induced Neuromechanical Pain Threshold in Lower Extremity of Diabetic RatsYAN Heguo1HOU Jianting2YIN Chanlan1YANG Bo1CHEN Wangli1LI Jun2*

1.Zhaotong hospital of traditional Chinese medicine，Zhaotong 657000，China;

2.Kunming hospital of traditional Chinese medicine，Kunming 650011，ChinaAbstract：Objective to study the effects of tangzhouling capsule on stz-induced neuromechanical pain threshold in lower extremity of diabetic rats.Methods 60 male SD rats were randomly divided into a normal control group and a modeling group after 1 week of adaptive feeding，including a normal group （10 rats），a modeling group （10 rats），a diabetes group，a low-dose group，a medium-dose group，a high-dose group，and a vitamin c group.After the model was successfully constructed，diabetes was administered to the tangzhouling group at a dose of 0.44g/（kg·d）.The medium dose group was given 0.88g/（kg·d）.The high-dose group was given 1.75g/（kg·d），the vitamin C group was given 0.05g/（kg·d），and the diabetes group and the control group were given the same amount of distilled water for 16 weeks. After 16 weeks，the mechanical pain threshold was measured with the Von Frey meter，and the effects of tangzhouling capsule on the mechanical pain threshold of the lower limbs of diabetic rats were observed.Results there were significant differences between normal control group and vitamin C group （P<0.05）.There were significant differences between normal control group，model group and small dose group （P<0.01）.There were significant differences between the model group and the low-dose group，the medium-dose group and the high-dose group （P<0.01）. Compared with the vitamin C group，there were significant differences between the low-dose group，the medium-dose group and the high-dose group （P<0.05）.Conclusion in the diabetic model group，the mechanical pain threshold was the lowest，tangzhouling tangzhouling capsule could improve the mechanical pain threshold of diabetic rats to some extent，improve the neurological function of diabetic neuropathy，and have a protective effect on diabetic peripheral neuropathy.

Keywords：Tangzhouling Capsule;Diabetes Mellitus;Mechanical Pain Threshold

糖尿病周围神经病变（Diabetic peripheral neuropathy，DPN）是指周围神经功能障碍，是糖尿病最常见的慢性并发症之一，亦是DPN中最常见的类型。研究报道我国DPN患病率为52.97%。DPN临床症状[1]早期常表现肢体麻木、疼痛、感觉异常等，并随着糖尿病病程逐渐进展[2-3]，可出现运动神经、感觉神经、自主神经均可受累，常表现为感觉缺失、腱反射消失，同时也可出现其他并发症。现代医学对DPN发病机制尚不明确，糖尿病并发症统一机制学说认为：高糖状态引起线粒体中超氧阴离子生成过多，从而导致组织细胞发生氧化应激是糖尿病周围神经病变发病机制[4-5]。西医对DPN的治疗主要是针对病因治疗和针对神经病变的发病机制治疗，针对病因治疗包括积极严格地控制高血糖并保持血糖稳定和使用甲钴胺、神经生长因子进行神经修复，针对神经病变的发病机制治疗包括抑制醛糖还原酶抑制剂、多元醇通路增强等对症治疗[6-8]。……