齐禹哲，闫芳芳，李成军，刘苏，陈鹏，徐传涛，李茂业，刘明科，闫雪梨，李斌

摘  要：為筛选出对烟草甲2龄幼虫具有较好防治效果的病原真菌，在实验室条件下，采用浸渍法测定5株蜡蚧轮枝菌和5株球孢白僵菌对烟草甲2龄幼虫的毒力，筛选出2株对烟草甲2龄幼虫具有高毒力的白僵菌菌株Bb05和Bbr81，将2株菌株配置成不同浓度的孢子悬浮液，测定其毒力并对Bb05菌株的侵染过程进行观察。结果表明，接种孢子浓度1.0×108 mL-1条件下，菌株Bb05处理烟草甲的累积死亡率最高，达77.78%，LT50为8.18 d;菌株Bbr81处理烟草甲的累积死亡率为74.44%，LT50为7.60 d。用不同浓度孢子悬浮液处理烟草甲2龄幼虫后，其累积死亡率随孢子浓度和接种时间的增加而增加，Bb05菌株的LC50为8.3×107 mL-1，菌株Bbr81的LC50为1.2×107 mL-1。筛选所得的2个菌株对烟草甲虫2龄幼虫的毒力较高，具有一定的开发应用潜力。

关键词：昆虫病原真菌;烟草甲;生物测定;生物防治

Screening for Highly Virulent Entomopathogenic Fungi Against Lasioderma serricorne （Fabricius） Larvae

QI Yuzhe1， YAN Fangfang2， LI Chengjun3， LIU Su1， CHEN Peng4， XU Chuantao5，

LI Maoye1*， LIU Mingke1， YAN Xueli1， LI Bin4*

（1. Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Institutes， School of Plant Protection， Anhui Agricultural University， Hefei 230036， China; 2. Sichuan Panzhihua Branch Tobacco Company， Panzhihua， Sichuan 617026， China; 3. Tobacco Research Institute of Henan Academy of Agricultural Sciences， Xuchang， Henan 464000， China; 4. Sichuan Branch of China National Tobacco Corporation， Chengdu 630041， China; 5. Luzhou Company of Sichuan Tobacco Company， Luzhou， Sichuan 646600， China）

Abstract： In order to screen for entomopathogenic fungi with better control effect on the second instar larvae of Lasioderma serricorne， in this experiment， 5 strains of Verticillium lecanii and 5 strains of Beauveria bassiana were determined by the impregnation method. Two Beauveria bassiana strains Bb05 and Bbr81 with high virulence to the second instar larvae of Lasioderma serricorne were obtained， and the two strains were prepared into different concentrations of spore suspension， and their virulence was determined. The infection process of the Bb05 strain was observed. The results showed that， at the concentration of 1.0×108 mL-1， Bb05 had the highest cumulative death rate to L. serricorne （77.78%） and LT50 （8.18 d）. The cumulative mortality of strain Bbr81 and LT50 was 74.44% and 7.60 d respectively. After treating the Lasioderma serricorne with different concentrations of spore suspensions， the cumulative mortality increased with the increase of spore concentration and inoculation time. The LC50 of strain BB05 was 8.3×107 spores mL-1， and that of strain Bbr81 was 1.2×107 spores mL-1. The two strains were highly virulent to the 2nd instar larvae of tobacco beetle， and have a certain potential for development and application.

Keywords： entomopathogenic fungi; Lasioderma serricorne; bioassay; biological control

烟草甲[Lasioderma serricorne （Fabricius）]又名烟草窃蠹，属鞘翅目（Coleoptera），窃蠹科（Anobiidae），具有适应能力强、食性广泛、产卵量大等特点，危害和污染各个加工阶段的烟草产品。此虫特别喜欢蛀食醇化1～2年的烟草叶片，其幼虫常隐匿于烟叶的夹层褶皱中取食烟叶，造成烟叶穿孔破碎，同时其虫尸及虫粪污染烟叶，严重影响烟叶品质[1-2]。目前对烟草甲的防治主要依靠磷化氢熏蒸，但随着烟草甲对磷化氢的抗性逐渐增强，其熏蒸效果大幅度降低[3-6]。同时，化学熏蒸极易造成环境污染，因此，迫切需要寻找对环境友好且不易产生抗性的防治药剂，以替代化学熏蒸。

昆虫病原真菌具有不污染环境、可持续控制害虫、易工业化生产等特点，在农林业害虫防治中具有巨大的应用价值。……