谭菲菲，高政绪，高强，时军，赵玉松，陈丹，林勇

摘  要：為探究氟吡菌酰胺及其代谢物AE C656948-Benzamide在烟草种植和加工过程中的残留降解特征，建立了QuEChERS-超高效液相色谱-串联质谱（QuEChERS-UPLC-MS/MS）法检测烟叶中氟吡菌酰胺及其代谢物残留量。结果表明，在0.05～10 mg/kg添加水平下，氟吡菌酰胺在鲜烟叶和干烟叶中的平均回收率分别为86%～94%和81%～95%，AE C656948-Benzamide在鲜烟叶和干烟叶中的平均回收率分别为85%～91%和83%～89%，相对标准偏差（RSD）均<10%，定量限（LOQ）均为0.05 mg/kg。氟吡菌酰胺在田间前3 d内消解率>50%，后期消解缓慢。烘烤过程中，氟吡菌酰胺的消解率为58.1%，AE C656948-Benzamide的消解率为52.9%，加工因子（Pf）为0.42～0.47。陈化360 d，氟吡菌酰胺的消解率为1.8%，AE C656948-Benzamide未发生降解，Pf为0.99～1.54。总之，烟叶中的氟吡菌酰胺和AE C656948-Benzamide在烟草种植和烘烤过程中降解明显，在陈化过程降解甚少。

关键词：氟吡菌酰胺;AE C656948-Benzamide;烟草;残留降解;加工因子

Residue Degradation of Fluopyram and its Metabolites during Tobacco Planting and Processing

TAN Feifei1， GAO Zhengxu2， GAO Qiang3， SHI Jun4， ZHAO Yusong4， CHEN Dan1*， LIN Yong5*

（1. Institute of Tobacco Research of CAAS， Qingdao 266101， China; 2. Shandong Weifang Tobacco Co.，Ltd.， Weifang， Shandong 261061， China; 3. Shandong Linyi Tobacco Co.，Ltd.， Linyi， Shandong 276003， China; 4. Pingdu Branch of Shandong Qingdao Tobacco Co.， Ltd.， Pingdu， Shandong 266700， China 5. Zhenghe Branch of Nanping Tobacco Company， Zhenghe， Fujian 353600， China）

Abstract： To investigate the residue degradation of fluopyram and its metabolite AE C656948-Benzamide during tobacco planting and processing， the QuEChERS-ultra performance liquid chromatography-tandem mass spectrometry （QuEChERS-UPLC-MS/MS） method was adopted to detect the residues of fluopyram and its metabolite in tobacco leaves. At addition levels of 0.05-10 mg/kg， the results showed that the average recoveries of fluopyram in fresh leaves and cured leaves were 86%-94% and 81%-95%， AE C656948-Benzamide were 85%-91% and 83%-89%， with relative standard deviations （RSD） less than 10%. The quantitative limits for fluopyram and AE C656948-Benzamide in fresh leaves and cured leaves were both 0.05 mg/kg. The degradation rate of fluopyram was more than 50% in the first 3 days， but it was slow after then. During curing the degradation rate of fluopyram residue was 58.1% and the degradation rate of AE C656948-Benzamide residue was 52.9%. The processing factors （Pf） were in the range of 0.42-0.47. The degradation rate of fluopyram was 1.8% and AE C656948-benzamide was not degraded within 360 days during aging. The Pf varied in the range of 0.99-1.54. In conclusion， fluopyram and AE C656948 benzamide degrade significantly during tobacco planting and baking， but rarely during aging.

Keywords： fluopyram; AE C656948-Benzamide; tobacco; residue and degradation; processing factors

氟吡菌酰胺（fluopyram），化学名称为N-{2-[3-氯-5-（三氟甲基）-2-吡啶基]乙基}-α，α，α-三氟邻甲苯酰胺，是拜耳股份公司开发的一种作用于琥珀酸脱氢酶（succinate dehydrogenase， SDH）的吡啶基乙基苯甲酰胺类新型广谱和内吸性杀菌剂，通过破坏呼吸电子传递链中的复合物Ⅱ（琥珀酸脱氢酶）来抑制线粒体的功能[1]。该药剂对多种作物病害有较好的防治效果，如番茄、黄瓜、西瓜、香蕉、烟草根结线虫病，黄瓜白粉病等[2]。目前报道的氟吡菌酰胺残留检测方法主要有气相色谱法[3-4]，液相色 谱法[5]，气质联用法[6-7]，液质联用法[8-9]，免疫测试法[10]，涉及到的样品基质主要有粮食、蔬菜、水果等，但尚无氟吡菌酰胺及其代谢物（AE C656948-Benzamide）在烟草中残留检测方法的研究报道。为进一步明确氟吡菌酰胺在烟草种植和加工过程中的……