李静宇，蒲小明，蒲浩杰，孙金锴，张冬生，王继华

摘 要：梅片树作为一种传统中药—天然右旋龙脑提取的新资源，目前生产上正遭受炭疽病的严重威胁，为了有效防治梅片树炭疽病，本研究选择8种杀菌剂对病原菌进行了室内毒力测定与筛选。结果表明：8种杀菌剂对炭疽菌菌丝生长均有一定的抑制作用，其中25%吡唑醚菌酯悬浮剂、80%多菌灵可湿性粉剂和15%咪鲜胺微乳剂抑制效果最好，EC50值分别为0.039 mg/L、0.170 mg/L和0.260mg/L;而10%苯醚甲环唑水分散粒剂、68.8%噁酮锰锌水分散粒剂、70%甲基硫菌灵可湿性粉剂、80%代森锰锌可湿性粉剂和75%百菌清可湿性粉剂抑制作用较弱。该研究结果为田间梅片树炭疽病防治杀菌剂选择提供了参考和理论依据。

关键词：梅片树;炭疽病;杀菌剂;毒力测定

中图分类号：S482.2+6                 文献标志码：A

Determination of Indoor Virulence of Eight Fungicides against Meipian Tree Anthracnose

LI Jingyu1，3，PU Xiaoming2，PU Haojie4，SUN Jinkai1，3，

ZHANG Dongsheng5， WANG Jihua1，3*

（1Guangdong Key Laboratory for Crops Genetic Improvement/Crop Research Institute， Guangdong Academy of Agriculture Sciences， Guangzhou， Guangdong 510640， China; 2Plant Protection Research Institute， Guangdong Academy of Agricultural Sciences / Guangdong Provincial Key Laboratory of High Technology for Plant protection， Guangzhou 510640， China; 3 Yunfu Yuncheng District Agricultural Rural and Water Bureau， Yunfu，Guangdong 527300， China; 4Meizhou Academy of Agricultural and Forestry Sciences， Forestry Research Institute， Meizhou，Guangdong 514014， China）

Abstract： Meipian tree is a new resource for extracting natural D-norneol——a traditional Chinese medicine. It is suffering from anthracnose in production. To control anthracnose， the indoor virulence effects of eight fungicides on the pathogen of anthracnose were determined. The results showed that the eight fungicides all had certain inhibitory effects on the mycelial growth， among them，25% pyraclostrobin SC，80% carbendazim WP and 15% prochloraz ME had the best effects， and their EC50 values were 0.039、0.170 and 0.260 mg/L， respectively;10% difenoconazole WG，68.8% famoxadone mancozeb WG，70% thiophanate-methyl WP， 80% mancozeb WP and 75% chlorothalonil WP had weak effects. This study provides reference and theoretical basis for selecting fungicides to control anthracnose in Meipian tree production.

Key words： Meipian tree; anthracnose; fungicides; virulence determination

梅片树是阴香（Cinnamomum burmannii）的一种生理变型，是1984年中科院华南植物园在广东省梅州市内发现的一种天然右旋龙脑新资源[1]。右旋龙脑又称天然冰片，是一种重要的传统中药，用于疾病的预防和治疗有超过2000年的历史，具有开窍醒神，退热止痛的功效，也是许多传统中成药中的重要组成成分[1]。天然右旋龙脑资源已近枯竭，从而导致国际上天然右旋龙脑奇缺、价格昂贵[1]。并且通过化学手段得到的合成龙脑，在储存过程中会逐渐降解产生有毒性的异龙脑[2]。梅片树叶挥发油中右旋龙脑含量高达68%[3]，是生产天然冰片的优良原材料。然而，梅片树正受到炭疽病的严重威胁，主产地广东省梅州市……