翟静娟

摘    要：为探讨不同物理和化学处理对翅果油树种子萌发的影响，本研究对翅果油树种子分别给予变温、激光、超声波、H2O2和PEG处理，以无处理种子作为对照，测定种子萌发过程（3，6，9，12，15，18 d）中的相关酶活性以及丙二醛含量的变化。结果表明：不同处理对翅果油树种子萌发过程中酶活性和丙二醛含量的影响存在差異，主要表现在相关指标随着萌发时间的波动变化方面。其中，超氧化物歧化酶活性变异系数表现为激光>变温>H2O2>超声波>对照>PEG;过氧化物酶活性变异系数表现为H2O2>激光>超声波>变温>对照>PEG;过氧化氢酶活性变异系数表现为超声波>H2O2>激光>PEG>变温>对照;抗坏血酸过氧化物酶活性变异系数表现为变温>H2O2>超声波>对照>激光>PEG;异柠檬酸裂解酶活性变异系数表现为对照>激光>超声波>PEG>H2O2>变温;MDA含量变异系数表现为激光>变温>超声波>PEG>H2O2>对照。综上，不同物理和化学处理会影响翅果油树种子萌发过程中相关酶活性及丙二醛含量的变化，但其与翅果油树种子萌发的关系仍需要进一步研究。

关键词：翅果油树;种子;萌发;酶活性

中图分类号：Q945.34         文献标识码：A        DOI 编码：10.3969/j.issn.1006-6500.2021.10.003

Effects of Physical and Chemical Interventions for Enzyme Activities during Seed Germination of Elaeagnus mollis Diels

ZHAI Jingjuan

（Shuozhou Vocational Technology College， Shuozhou， Shanxi 036002， China）

Abstract： To analyze the effects of different physical and chemical interventions during the seed germination of Elaeagnus mollis Diels， seeds of Elaeagnus mollis Diels were dealt with variable temperature， laser， ultrasonic， H2O2， and PEG respectively， no interventions seeds were used for as control. The related enzymes activities and malonaldehyde content were measured during the seed germination （3， 6， 9， 12， 15， 18 d）. The results showed that the effects on enzyme activity and malondialdehyde content were various among different interventions during the seed germination of Elaeagnus mollis Diels， which mainly involved the fluctuation of related indexes following with the germination time. The variable coefficient of superoxide dismutase was manifested as laser>variable temperature>H2O2>ultrasonic>control>PEG; variable coefficient of peroxidase was manifested as H2O2>laser > ultrasonic > variable temperature>control>PEG; variable coefficient of catalase was manifested as ultrasonic>H2O2>laser>PEG>variable temperature>control; variable coefficient of ascorbate peroxidase was manifested as variable temperature>H2O2>ultrasonic>control>laser>PEG; variable coefficient of isocitrate lyase was manifested as control>laser>ultrasonic>PEG>H2O2>variable temperature; variable coefficient of MDA was manifested as laser>variable temperature>ultrasonic>PEG>H2O2>control. To sum up， different physical and chemical interventions may impact the related enzymes activities and malonaldehyde content during the seed germination of Elaeagnus mollis Diels， but further studies are needed to investigate the relationship between these interventions and seed germination of Elaeagnus mollis Diels.

Key words： Elaeagnus mollis Diels; seed; germination; enzymatic activity

翅果油树（Elaeagnus mollis Diels）是我国特有的一种乔木状灌丛或小乔木，属于国家Ⅱ级珍稀濒危的保护植物[1-2]。翅果油树具有一定的耐寒性和耐旱性，主要分布于山西省境内，在陕西秦岭等地也有散在分布[3-4]。翅果油树具有非常高的经济价值。翅果仁富含多种蛋白质、维生素、脂肪酸等，能够降低胆固醇、减少动脉粥样硬化等[5]。翅果油树叶中含有的总生物碱具有一定的抗氧化作用，在抗衰老方面具有重要价值[6]。权洪峰等[7]研究表明，翅果油树种子具有一定的抗炎和神经保护活性。但是，翅果油树的种子具有坚硬的外壳，透水性较差，导致翅……