多肉植物红宝石组织培养快繁技术
2021-10-28陈伟曾凡赵光辉
陈伟 曾凡 赵光辉



摘 要:为建立多肉植物红宝石高效组织培养快繁技术体系,为工厂化生产提供技术保障。以多肉植物红宝石的幼嫩叶片作为外植体,进行多肉植物红宝石组培快繁技术研究。结果表明:采用75%酒精浸泡60 s,再使用0.1%升汞处理10 min是红宝石外植體最佳消毒处理方法。红宝石愈伤组织诱导的最适培养基为MS+6-BA 1.5 mg·L1+NAA 0.5 mg·L1(pH=6.0),诱导率可达83.3%;愈伤组织分化成芽的最适培养基为MS+6-BA 0.6 mg·L1+NAA 0.2 mg·L1(pH=6.0),分化率可达77.78%;生根最适培养基为1/2 MS+活性炭2.0 g·L1(pH=6.0),生根率可达70.00%。
关键词:多肉植物;红宝石;组织培养;快繁技术
中图分类号:S 682.33 文献标志码:A 文章编号:0253-2301(2021)08-0033-06
DOI: 10.13651/j.cnki.fjnykj.2021.08.005
Rapid Propagation of Sedeveria Pink Ruby by Tissue Culture
CHEN Wei, ZENG Fan, ZHAO Guang-hui
(Fuzhou Institute of Agricultural Sciences, Fuzhou, Fujian 350018, China)
Abstract: In order to establish the rapid propagation technology system of Sedeveria pink ruby by high-efficient tissue culture, and provide the technical support for industrialized production, the tissue culture and rapid propagation technology of Sedeveria pink ruby was studied by using the young leaves of Sedeveria pink ruby as the explants. The results showed that 75% alcohol soaked for 60 s and 0.1% mercury bichloride treated for 10 min were the best disinfection treatments for the explants of Sedeveria pink ruby. The optimal culture medium for callus induction of Sedeveria pink ruby was MS+1.5 mg·L1 6-BA+0.5 mg·L1 NAA (pH=6.0), with the induction rate of 83.3%. The optimum culture medium for bud differentiation from callus was MS+0.6 mg·L1 6
-BA+0.2 mg·L1 NAA (pH=6.0), with the differentiation rate of 77.78%. The optimal culture medium for rooting was 1/2 MS+2.0 g·L1 activated carbon (pH=6.0), and the rooting rate could reach 70.00%.
Key words: Succulents; Sedeveria pink ruby; Tissue culture; Rapid propagation technology
多肉植物红宝石Sedeveria pink ruby为景天科拟石莲花属植物,是近几年发展较快的一种多肉植物[1]。红宝石是中小型多肉品种,非常容易群生,长成爆盆品种;其叶片较多,光滑,细长匙状,前端较肥厚、斜尖,呈莲花状紧密排列,整体较包裹,植株紧凑,单株直径5 cm左右。红宝石正常生长时颜色为绿色,如温差大、光照充足,其颜色会从绿色变成火红色,色泽如宝石,加上其容易群生,长成捧花状态,具有较高的观赏价值。但红宝石生产主要以叶插繁殖为主,因其繁殖速度慢、生产周期长、加上病毒不断积累,品质受到较大的影响。
植物组织培养是利用植物细胞能够发育成完整植株的功能进行的无性繁殖方法,组织培养能保持原有品种固有的性状和特性,可以用来解决制约多肉植物产业快速发展的繁殖问题[2-6]。目前已有景天属景天科大和锦、劳尔、石台景天、大花红景天、青锁龙、八宝景天、黄丽等多肉植物组织培养的研究[7-13],但是利用红宝石叶片作为外植体进行组织培养研究未见报道。……
