张维明 李立纪 陶剑 陈得玲 唐世朗

【摘 要】 目的：

探讨豆腐渣果的活性成分豆腐果苷的抗血栓和减轻脑内炎症因子释放的作用。方法：采用大鼠下腔静脉血栓模型和花生四烯酸颈内动脉注射诱发急性脑血栓形成模型，检测豆腐果苷干预花生四烯酸颈内动脉注射诱发急性脑血栓形成模型后，动物脑组织中炎症因子含量的情况，并对豆腐果苷抑制脑内炎症的作用机制进行了初步分析。结果：豆腐果苷对两种血栓模型均有明显的对抗作用，说明豆腐果苷在体内具有显著的抗血栓形成作用。结论：对花生四烯酸诱导的急性脑血栓动物模型，豆腐果苷能明显降低血脑屏障的通透性，且能降低炎症因子TNF-α、IL-1β的含量，其减轻急性脑血栓大鼠脑内炎症的作用机制与抑制花生四烯酸代谢脂氧酶途径的炎症反应有关。

【关键词】 豆腐果苷;抗血栓;抗炎

【中图分类号】R285.5   【文献标志码】 A    【文章编号】1007-8517（2021）24-0014-04

Anti-thrombosis and Alleviating Inflammatory Factors In Brain with Helicid

ZHANG Weiming1 LI Liji2 TAO jian1 CHEN Deling1 TANG Shilang1

1.School of Medicine，Kunming University，Kunming 650214，China;

2.Kunming Institute of Medical Sciences，Kunming 650041，China

Abstract：Objective To explore the effect on anti-thrombosis andalleviating the release of inflammatory factors in brain by the active ingredient of helicid in Helicia nilagirica Bedd，Rat venous thrombosis caused by inferior vena cava ligation method and acute cerebral thrombosis induced by infection of arachidonic acid via intracarotid artery method were used to evaluate the anti-thrombotic effect of helicid. Methods At the same time the mechanism of the inhibitory effect of helicid on brain inflammation was preliminarily analyzed.Resudts helicid had obvious antagonistic function on the two thrombosis models，which indicated that helicid had significant anti-thrombotic effect in vivo. Conclusion In the acute cerebral thrombosis model tests caused by the injection of arachidonic acid，helicid can significantly reduce the permeability of blood-brain barrier，and reduce the contents of inflammatory factors TNF-α and IL-1β.The mechanism of reducing inflammation in rats with acute cerebral thrombosis is related to inhibiting the inflammatory reaction of arachidonic acid metabolism lipoxygenase pathway.

Key words：Helicid;Anti-thrombotic;Anti- inflammatory

豆腐果苷（Helicid，結构式如图1所示）为山龙眼科植物深绿山龙眼（Helicia nilagirica Bedd）的干燥果实（豆腐渣果）的活性成分之一，豆腐渣果广泛分布于云南西南部和南部（西双版纳、普洱、保山、红河）等地，资源丰富，在云南西双版纳地区是独具民族特色和地区特色的傣医常用解毒药，可用于解农药和食物中毒[1]。目前对豆腐渣果的研究集中于其主要活性成分豆腐果苷，豆腐果苷是一种天然阿洛糖酚苷类化合物，其独特性在于结构中的糖基为稀有的吡喃阿洛糖，临床及药理活性研究[2]表明，豆腐果苷具有肯定的止痛、镇静和催眠作用，对神经衰弱综合征有较好的疗效，目前已开发出以之为主要成分的药物“神衰果素片”上市并销售，临床主要用于缓解神经官能症的头痛、头昏及睡眠障碍。……