胡积祥 朱秀梅 余超 杨凤环 王继春 张立新 陈华民

摘要 水稻白叶枯病是由稻黄单胞菌水稻致病变种Xanthomonas oryzae pv. oryzae（Xoo）引起的一种细菌性病害，严重影响水稻的产量和品质。miR1320是水稻与Xoo互作中重要的调控因子。本研究在接种Xoo后的0、12、24 h分别检测了10个粳稻和8个籼稻品种中miR1320的积累和4个防卫基因的转录水平，测量了各品种叶片上的病斑长度，分析了miR1320的积累和防卫基因的表达与水稻白叶枯病抗性之间的相关性。结果表明：接种Xoo后，miR1320的表达模式在不同品种中有所不同;籼稻和粳稻对白叶枯病的抗性分别与接种后24 h和12 h時miR1320的表达量呈紧密负相关;且相对于防卫基因，miR1320更能反映品种间的抗病性。因此，miR1320可应用于评估水稻品种对白叶枯病的抗性，辅助水稻抗病种质的鉴定。

关键词 miR1320; 水稻; 白叶枯病; 抗性; 表达

中图分类号： S 435.11

文献标识码： A

DOI： 10.16688/j.zwbh.2020354

Analysis of the correlation between the expression level of miR1320 and resistance to bacterial blight in rice

HU Jixiang1， ZHU Xiumei1， YU Chao1， YANG Fenghuan1，

WANG Jichun2， ZHANG Lixin3， CHEN Huamin1*

（1. State Key Laboratory for Biology of Plant Disease and Insect Pests， Institute of Plant Protection， Chinese

Academy of Agricultural Sciences， Beijing 100193， China; 2. Institute of Plant Protection， Jilin Academy

of Agricultural Sciences， Gongzhuling 136100， China; 3.College of Plant Protection，

Anhui Agricultural University， Hefei 230036， China）

Abstract

Rice bacterial blight （BB）， caused by Xanthomonas oryzae pv. oryzae （Xoo）， is a serious bacterial disease and significantly reduces the rice yield and quality in the field. miR1320 is an important regulatory factor in the interaction between rice （Oryzae sativa） and Xoo. In this study， the expression levels of miR1320 and four defense genes were detected in 10  japonica rice （Oryzae sativa subsp. geng） and eight indica rice （Oryzae sativa subsp. xian） varieties at 0、12、24 h time points after inoculation with Xoo. Then the leaf lesion length was measured on the 14th day after inoculation with Xoo. Finally， the correlations between lesion length and either miR1320 or defense genes were analyzed. The results suggested that the different rice varieties displayed different patterns in response to Xoo infection， and the resistance to BB in japonica rice and indica rice was closely and negatively correlated with the expression levels of miR1320 at 24 hpi （hours post inoculation） and 12 hpi， respectively. Moreover， compared with defense genes， miR1320 was a better indicator of the resistance to BB in different rice varieties. In conclusion， miR1320 can be applied to assess the resistance of rice varieties to BB and assist the identification of rice resistant germplasms.

Key words

miR1320; rice; bacterial blight; resistance; expression

水稻白叶枯病是由稻黄单胞菌水稻致病变种Xanthomonas oryzae pv. oryzae（Xoo）侵染引起的一种细菌病害，对水稻产量影响轻的减产20%～30%，重的可达50%～60%，甚至颗粒无收。病株的稻谷加工后碎米多，严重降低稻米的品质[1-2]。在生产上，尽管喷施杀菌剂能在一定程度上控制病害的发生，但杀菌剂的使用严重污染了当地的环境，大大增加了水稻的生产成本。因此，种植抗病品种是防治水稻白叶枯病最经济有效且绿色环保的策略[3]，而作物抗病性鉴定是作物抗病育种的重要基础性环节，快速的鉴定手段可以提高育种效率、加快育种进度。

miRNA是生物体内源性存在的、长度为21～24个核苷酸的……