周嘉培 王小平 曹志群 王永森

摘要 目的：借助网络药理学平台，研究半夏泻心汤治疗功能性消化不良（FD）的作用靶点及作用机制。方法：基于中药系统药理学数据库与分析平台（TCMSP）筛选半夏泻心汤的有效成分及作用靶点。通过GeneCards数据库提取FD相关基因。运用Cytoscape软件构建“活性成分-靶点-疾病”网络图，上传关键靶点至STRING平台建立蛋白质-蛋白质相互作用网络，最后利用ClueGO插件进行京都基因与基因组百科全书（KEGG）通路富集分析。结果：半夏泻心汤有效活性成分共211种;关键靶点共70个，包括ALB、CASP3、VEGFA等;KEGG通路富集得到相关作用通路15类，主要涉及TNF信号通路、核因子κB信号通路、5-羟色胺（5-HT）信号通路、PI3K-AKT信号通路等。结论：调节炎症、胃动力、胃肠高敏性以及雌激素含量等途径是半夏泻心汤治疗FD的潜在作用机制，本研究为FD治疗方案的进一步改进提供了客观的参考方向。

关键词 半夏泻心汤;功能性消化不良;作用机制;网络药理学;炎症;胃动力;内脏高敏性;雌激素水平

Internet Pharmacology Study of Banxia Xiexin Decoction in the Treatment of Functional Dyspepsia

ZHOU Jiapei1，WANG Xiaoping1，CAO Zhiqun2，WANG Yongsen2

（1 School of Traditional Chinese Medicine，Shandong University of Traditional Chinese Medicine，Ji′nan 250355，China; 2 Digestive Endoscopy Clinic Department，Affiliated Hospital of Shandong University of Traditional Chinese Medicine，Ji′nan 250014，China）

Abstract Objective：To study the related target and mechanism of Banxia Xiexin Decoction in the treatment of functional dyspepsia by using network pharmacology.Methods：The active ingredients and targets of Banxia Xiexin Decoction were screened by TCMSP.Functional Dyspepsia related genes were extracted from the Genecards database.The network map of “active ingredient-target-disease” was constructed by Cytoscape software.The key targets were uploaded to the STRING platform to establish the protein interaction network.Finally，the ClueGO plug-in was used to analyze the enrichment of the KEGG pathway.Results：There were 211 active ingredients in Banxia Xiexin Decoction; a total of 70 key targets，including ALB，CASP3，VEGFA，EGFE，IL6 and MAPK8.The enrichment of KEGG pathway resulted in 15 types of related pathways，mainly including TNF signaling pathway，NF-kappa B signaling pathway，5-HT signaling pathway and p13k-art signaling pathway.Conclusion：Regulating inflammation，gastric motility，gastrointestinal hypersensitivity，estrogen content and other approaches are the potential mechanisms of Banxia Xiexin Decoction in the treatment of FD.This study provides an objective reference direction for the further improvements of FD treatment.

Keywords Banxia Xiexin Decoction; Function Dyspepsia; Action mechanism; Network pharmacology; Inflammation; Gastric motility; Visceral hypersensitivity; Estrogen level

中图分类号：R285文献标识码：Adoi：10.3969/j.issn.1673-7202.2021.17.010

功能性消化不良（Functional Dyspepsia，FD）为一组非器质性、系统性、代谢性疾病的功能性症候群，以上腹部疼痛或烧灼感、餐后饱胀不适以及早饱为主症，还可伴有食欲不振、嗳气、恶心或呕吐等兼夹症状[1]，临床发病率高，普通人群发病率可达20%～40%，且有逐年上升的趋势[2]。FD的现代医学机制尚未明确，目前认为其发病主要与胃肠道运动功能障碍、内脏高敏性、胃酸刺激、幽门螺杆菌（Helicobacter Pylori，HP）感染以及精神心理因素等因素相关[3]。单纯服用西药治疗效果有限，复发率高，对患者生命质量亦有较大影響。……