铁观音与荆芥提取物协同抗氧化作用研究
2021-09-10庞海月黄立森陈煜沛吴黉坦陈芳芳王贵弘
庞海月 黄立森 陈煜沛 吴黉坦 陈芳芳 王贵弘


摘要:为研究铁观音提取物和荆芥提取物联用在抗氧化方面的协同作用,采用水浸提法分别萃取铁观音和荆芥得其抗氧化活性成分。以芦丁为标准品检测两种提取物类黄酮含量,其中铁观音提取物为15.13 mg/g,荆芥提取物为0.87 mg/g;以没食子酸为标准品检测两种提取物的总酚含量,其中铁观音水提物为50.87 μg/mg,荆芥水提物为47.40 μg/mg。用2,2-二苯基-1-苦基肼(DPPH)和2,2'-二氮-双(3-乙基苯并噻唑-6-磺酸)(ABTS)法评估两种提取物联合应用时的抗氧化活性,结果表明,在较低浓度时,铁观音与荆芥提取物联用具有协同增强抗氧化作用。
关键词:协同作用;抗氧化;2,2-二苯基-1-苦基肼;2,2'-二氮-双(3-乙基苯并噻唑-6-磺酸)
Study on the Synergistic Antioxidant Effect of
Tieguanyin and Schizonepeta Extracts
PANG Haiyue1,2, HUANG Lisen1,2, CHEN Yupei1,2, WU Hongtan1,2, CHEN Fangfang1,2, WANG Guihong1,2*
1. Department of Public Health and Medical Technology, Xiamen Medical College, Xiamen 361023, China;
2. Engineering Research Center of Natural Cosmeceuticals College of Fujian Province, Xiamen 361023, China
Abstract: In order to study the synergistic effect of Tieguanyin and Schizonepeta extracts on antioxidation, water extraction method was used to extract the active components. The contents of flavonoids in the two water extracts were determined by rutin method, and the contents of total phenols in the water extracts were 15.13 mg/g (Tieguanyin) and 0.87 mg/g (Schizonepeta Tenuifolia) respectively. The contents of total phenols in the water extractswere determined by gallic acid assay, and the water extracts were 50.87 μg/mg (Tieguanyin) and 47.40 μg/mg (Schizonepeta Tenuifolia). The DPPH and ABTS methods were applied to assess the antioxidant activities of the two extracts. The results show that the combined use of Tieguanyin and Schizonepeta Tenuifolia extracts had syntheticallyenhanced the antioxidant effect.
Keywords: synergistic effect, antioxidant, DPPH, ABTS
饮茶是我国一种悠久的饮食文化,受到人民群众的普遍喜爱[1]。茶之所以广受欢迎,一是饮茶能使人身心振奋愉悦,二是受茶文化的熏陶,饮茶长期作为养生、休闲和交流的一种媒介[2]。现代科学研究也表明饮茶确实有益健康,这与茶叶中含有的茶多酚、咖啡碱、脂多糖、维生素、氨基酸等成分有关[3],科学饮茶具有延缓衰老、抗辐射、预防肿瘤和心血管疾病、美容、抗菌、降脂、护齿等功效[4]。
许多文献报道铁观音具有良好的抗氧化功能,这与其含有茶多酚、儿茶素、没食子酸、黄酮等多种抗氧化物质有关。荆芥是一种多年生唇形科草本植物,具有解表散风、透疹等功效,为临床治疗感冒、头痛、麻疹、风疹、疮疡等疾病处方组成成分[5],在民间也常用作泡茶和烹饪佐料,研究还表明荆芥具有一定的护胃功效。……
