杨盺宇

【摘要】目的：探讨欧亚旋覆花总黄酮（total flavones extracted from Inula britannica L，TFIB）对肢体缺血再灌注骨骼肌的保护作用。方法：健康成年家兔20只，随机分为对照组和实验组，每组10只，建立兔肢体缺血再灌注损伤动物模型。在即将恢复血流灌注时，两组分别自耳缘静脉注射生理盐水（对照组）、欧亚旋覆花总黄酮注射液（实验组）。分别在缺血前、缺血后2h、再灌注后1h、再灌注后3h采集术侧股静脉血样。测定血清乳酸脱氢酶（LDH）、肌酸激酶（CK）的含量。取上述各时相点动物胫前肌组织，测定骨骼肌组织湿/干重比值并观察肌组织微细和超微结构变化。结果：实验组再灌注后LDH、CK明显低于对照组（P<0.01），骨骼肌组织湿/干重比值低于同期对照组（P<0.05）。光镜及电镜下观察实验组骨骼机损伤轻于对照组。结论：欧亚旋覆花总黄酮能够缓解组织水肿，保护缺血再灌注骨骼机。

【关键词】欧亚旋覆花总黄酮;骨骼肌;再灌注损伤

【中图分类号】R681;【文献标识码】A; 【文章编号】2026-5328（2021）02-025-05

Abstract：Objective：To study the protective effect and mechanism of Total flavones extracted from Inula britannica L （TFIB）on ischemia reperfusion injury of skeletal muscle. Methods：The experimental model of ischemia reperfusion injury in extremity were produced in 20 healthy adult rabbits which were randomly divided

into experimental and control groups（each of 10 rabbits）. Normal saline injection and TFIB were infused intravenously into rabbits of control group，experimental group respectively just before blood reperfusion. At preischemia，2 hours post-ischemia，1 hour post-reperfusion and 3 hours post-reperfusion，sample of blood was collected from the femoral veins respectively for measurements of lactic dehydrogenase（LDH），creatine kinase（CK） by means of biochemical methods in every rabbit. Meanwhile the tibialis anterior muscles were harvested for the measurements of Wet/Dry ratio of skeletal muscle and for the microscope and ultrastructure examinations by using optical microscope and electronic microscope respectively . Results ：After reperfusion，the contents of LDH、CK were dramatically higher in control group（P<0.01） but were not in experimental group（P>0.05）. The Wet/Dry ratio of skeletal muscle had not obvious difference in every group（P>0.05） before reperfusioin. The skeletal muscle injury of experimental groups，after reperfusion was of a mild degree than of control group in light microscope and electronic microscope examinations. Conclusion：TFIB can effectively improve soft tissue swelling and protec skeletal muscle from ischemia reperfusion injury.

Key words：Total flavones extracted from Inula britannica L;Skeletal muscle; Reperfusion injury

肢体的创伤、断肢再植、血栓形成、组织移植以及止血带的应用时间过长均可引起肢体缺血。随着血流的恢复，在随后的一段时间内组织损伤不仅不减轻反而逐渐加重，这种现象称为肢体缺血再灌注损伤。它不仅影响缺血肢体组织的存活及功能，而且可累及全身多器官，严重时可引发多器官功能衰竭而导致患者死亡[1，2]。欧亚旋覆花是菊科旋覆花属，多年生草本植物，性味咸、温;入肺、肾经。其黄酮类物质的含量高达10.9%，黄酮类化合物具有清除自由基和抗应激反应的能力。……