张明 梁志云 张俊杰 张健民 余香 昌水平 蒋三元

【摘 要】 目的：建立消瘀跌打药酒制剂和其主药通城虎药材中马兜铃酸I的含量测定方法。方法：采用高效液相色谱法。选用Inertsil ODS-XP（4.6 mm×150 mm，5μm）色谱柱，甲醇-0.05%冰醋酸溶液（53∶47）为流动相洗脱，检测波长：250 nm，流速：1 mL·min-1，柱温：30 ℃。结果：马兜铃酸I的进样浓度在3.884～38.84 μg·mL-1范围内与峰面积积分值呈良好的线性关系，回归方程Y=55825X-12839（r=0.9999）;消瘀跌打药酒中马兜铃酸I的回收率平均为99.08%，RSD为0.61%;通城虎中马兜铃酸I的回收率平均为98.65%，RSD为1.58%。结论：建立的消瘀跌打药酒制剂和通城虎药材中马兜铃酸I含量测定方法，简便，重现性良好，可用于药材与制剂的质量控制。

【关键词】 消瘀跌打药酒;通城虎;马兜铃酸I;成分分析

【中图分类号】R286 【文献标志码】 A 【文章编号】1007-8517（2021）14-0044-03

Abstract：Objcetivet To establish the method for the content determination of aristolochic acid I in Xiaoyudieda medicinal liquor and Aristolochia fordiana Hemsl. Methods HPLC method.Chromatographic conditions were as follows： Inertsil ODS-XP（4.6 mm×150 mm，5 μm）column was used with mobile phase consisted of methanol-0.05%glacial acetic acid（53∶47） at a flow rate of 1.0 mL·min-1 under a detection wavelength at 250 nm. The column temperature was 30 ℃. Results The linear range of aristolochic acid I was 3.884～38.84 μg·mL-1，and the regression equation was Y=55825X-12839（r=0.9999）.The average recovery rate of aristolochic acid I in Xiaoyudieda medicinal liquor was 99.08%，RSD was 0.61%.The average recovery rate of aristolochic acid I in Aristolochia fordiana Hemsl was 98.65%，RSD was 1.58%. Conclusion The method established in the experiment can be used to determine the content of aristolochic acid I in Xiaoyudieda medicinal liquor and Aristolochia fordiana Hemsl. There were significant differences in aristolochic acid I content between different producing areas and different batches.

Keywords：Xiaoyudieda Medicinal Liquor;Aristolochia fordiana Hemsl; Aristolochic acid I ;Content Determination

通城虎，又名大力王藤、五虎通城、定心草等，為马兜铃科马兜铃属植物通城虎 Aristolochia fordiana Hemsl.的根或全株，其味苦、辛，性温，有小毒，有解毒消肿、祛风镇痛、行气止咳之功[1]，是两广及香港地区常用中草药，在广东新会地区民间被广泛使用，常用于胃痛、风湿骨痛、跌打损伤、毒蛇咬伤等。通城虎是我院传统制剂消瘀跌打药酒中的主要药材之一，该制剂具有驱风活血、散瘀止痛的功效，用于跌打腰痛、扭挫伤。通城虎含马兜铃酸类成分可在体内经一系列硝基还原酶代谢活化后与DNA共价结合形成加合物而诱发癌变，并通过诱导肾小管上皮细胞凋亡及间质纤维化而导致“马兜铃酸肾病 ”（AAN）[3]。马兜铃酸主要含有马兜铃酸I和马兜铃酸II两种化合物，其中前者是马兜……