黄小芳 毕楚韵 王和寿 陈其俊 胡韵卓 陈选阳 林世强

摘  要：利用生物信息學方法对甘薯（Ipomoea batatas）基因组中NAC转录因子进行鉴定、保守结构域分析、motif查找、染色体定位、系统进化树分析以及逆境胁迫下基因表达分析。结果表明：甘薯全基因组序列中含有91个NAC转录因子基因，非均匀分布于甘薯15条染色体上;系统进化树及motif分析结果显示，91个甘薯NAC家族成员可分为16个亚组，共包含20个motif，其中大部分家族成员中均含有motif 2、motif 4、motif 1、motif 8和motif 3，这5个motif分别对应NAC结构域中的5个子域A、B、C、D和E。在与拟南芥（Arabidopsis thaliana）NAC转录因子共同构建的进化树中，有64个甘薯NAC家族成员被归入拟南芥NAC基因家族的14个亚组，其中，NAC2亚组包含的成员数最多，有9个，TIP和AtNAC3亚组均仅有1个。转录组数据分析结果显示，在蔓割病菌（Fusarium oxysporum f. sp. batatas， Fob）胁迫下甘薯NAC基因家族中有10个基因的表达量发生变化;而在低温环境下有25个NAC基因差异表达。本研究结合利用基因组与转录组数据分析，该结果为进一步研究甘薯NAC基因家族的功能提供参考。

关键词：甘薯;NAC;转录因子;生物信息学

中图分类号：S531      文献标识码：A

Genome-wide Identification and Analysis of NAC Transcription Factor Family of Ipomoea batatas

HUANG Xiaofang1，2， BI Chuyun1，2， WANG Heshou3， CHEN Qijun4， HU Yunzhuo1， CHEN Xuan-yang1，2，5*， LIN Shiqiang1，6*

1. Key Laboratory of Crop Biotechnology， Fujian Agriculture and Forestry University， Fuzhou， Fujian 350002， China; 2. College of Agriculture， Fujian Agriculture and Forestry University， Fuzhou， Fujian 350002， China; 3. Ningde Agricultural and Rural Bureau， Ningde， Fujian 352100， China; 4. Seed Centre of Fujian， Fuzhou， Fujian 350002， China; 5. Key Lab of Genetics， Breeding and Multiple Application of Crops， Ministry of Education， Fujian Agriculture and Forestry University， Fuzhou， Fujian 350002， China; 6. College of Life Science， Fujian Agriculture and Forestry University， Fuzhou， Fujian 350002， China

Abstract： The bioinformatic methods were used to conduct the identification， conservative domain analysis， motif search， chromosomal localization， phylogenetic analysis and gene expression analysis under stress with regard to the NAC transcription factors of the Ipomoea batatas genome. The results showed that there existed 91 genes of NAC transcription factors within the I. batatas genome， which were distributed unevenly in the 15 chromosomes. Phylogenetic and motif analysis indicated that the 91 members of NAC transcription factors could be classified into 16 subgroups and there were 20 motifs in total. Most members of the NAC family contained motif 2， motif 4， motif 1， motif 8 and motif 3， which corresponded to the subdomains A， B， C， D and E of the NAC domain. Within the phylogenetic tree constructed with the transcription factors of I. batatas and Arabidopsis thaliana， there were 64 I. batatas NAC transcription factors categorized into the 14 subgroups of A. thaliana NAC transcription factors， of which the NAC2 subgroup was the largest in number and the TIP subgroup and AtNAC3 subgroup both had only one member. Analysis of the transcriptomics data demonstrated that 10 genes of the I. batatas NAC transcription factors were differentially expressed under Fusarium oxysporum f. sp. batatas stress; whereas under the low temperature condition， there were 25 NAC genes differentially expressed. This study combined the analysis of genomic and transcriptomics data of I. batatas and the results provided reference for the functional inquiry into the I. batatas NAC transcription factors.

Keywords： Ipomoea batatas; NAC; transcription factor; bioinformatics

DOI： 10.3969/j.issn.1000-2561.2021.07.004

近几年来，对植物转录因子的研究越来越广泛。转录因子根据其DNA结合结构域的不同可分为WRKY、MYB、bHLH、bZIP和NAC等多个转录因子家族[1]。NAC转录因子是植物中特有的一类转录因子超家族，最早在矮牵牛和拟南芥中发现，Aida等[2]研究发现矮牵牛NAM基因和拟南芥ATAF1、ATAF2以及CUC2的3个基因所编码的蛋白序列N端均有一段高度保守的氨基酸序列，因此将这一段高度保守的区域命名为NAC结构域。典型的NAC转录因……