祝雪丽 熊尚全 陈怀宇 褚剑锋 吴翔 何绍吾 李玮

摘要 目的：研究丹參饮通过介导过氧化物酶体增殖物激活型受体γ（PPAR-γ）/肝X受体α（LXR-α）/三磷酸腺苷结合盒转运体A1（ABCA1）信号通路减轻大鼠冠状动脉粥样硬化的作用机制。方法：选取无特定病原体（SPF）级40只健康Wistar大鼠作为实验动物，随机分为空白组、冠状动脉粥样硬化组（对照组）、丹参饮低剂量观察组（低剂量组）和丹参饮高剂量观察组（高剂量组），比较各组大鼠的炎症介质水平、ICAM-1水平、脂代谢水平、PPAR-γ、LXR-α和ABCA1 mRNA表达和蛋白表达。结果：对照组、低剂量组和高剂量组的三酰甘油（TG）、胆固醇（TC）、高密度脂蛋白胆固醇（HDL-C）和低密度脂蛋白胆固醇（LDL-C）均高于空白组（P<0.05），低剂量组和高剂量组的TG、TC和LDL-C均低于对照组（P<0.05），且高剂量组低于低剂量组（P<0.05），但4组的HDL-C表达差异无统计学意义（P>0.05）;对照组、低剂量组和高剂量组的炎症介质和ICAM-1表达均高于空白组（P<0.05），低剂量组和高剂量组的炎症介质和ICAM-1表达均低于对照组（P<0.05），且高剂量组低于低剂量组（P<0.05）;对照组、低剂量组和高剂量组的PPAR-γ、LXR-α和ABCA1 mRNA和蛋白表达均高于空白组（P<0.05），且低剂量组和高剂量组明显高于对照组（P<0.05），高剂量组明显高于低剂量组（P<0.05）。结论：丹参饮可以通过对PPAR-γ-LXR-α-ABCA1信号通路的介导，发挥出抗冠状动脉粥样硬化的作用。

关键词 丹参饮;冠状动脉粥样硬化;过氧化物酶体增殖物激活型受体γ;肝X受体α;三磷酸腺苷结合盒转运体A1;丹参;檀香;砂仁;冠心病预防

Abstract Objective：To study on Danshen Drink by mediating peroxisome proliferator-activated receptor γ（PPAR-γ）/liver X receptor α（LXR-α）/adenosine triphosphate binding cassette transporter A1（ABCA1） signaling pathway to alleviate the mechanism of coronary atherosclerosis of rats.Methods：A total of 40 healthy Wistar rats of SPF-free grade were selected as the experimental animals，and were randomly divided into a blank group，a coronary atherosclerosis group（control group），Danshen Drink low-dose treatment group（low-dose group） and Danshen Drink high Dose treatment group（high-dose group）.The levels of inflammatory mediators，ICAM-1，lipid metabolism，PPAR-γ，LXR-α and ABCA1 mRNA expression and protein expression in each group of rats were compared.Results：The triacylglycerol（TG），cholesterol（TC），high-density lipoprotein cholesterol（HDL-C） and low-density lipoprotein cholesterol（LDL-C） of the control group，low-dose group and high-dose group were all higher than those of the blank group（P<0.05），the TG，TC and LDL-C of the low-dose group and the high-dose group were lower than those of the control group（P<0.05），and the high-dose group was lower than the low-dose group（P<0.05），but there was no statistically significant difference in the HDL-C expression of 4 groups（P>0.05）; the expressions of inflammatory mediators and ICAM-1 in the control group，low-dose group and high-dose group were higher than those in the blank group（P<0.05），low-dose group and high-dose group The expressions of inflammatory mediators and ICAM-1 in the control group were lower than those in the control group（P<0.05）.The protein expressions of PPAR-γ、LXR-α and ABCA1 mRNA were higher than those of the blank group（P<0.05）.And the low-dose and high-dose groups were significantly higher than those of the control group（P<0.05），and the high-dose group was significantly higher than the low-dose group（P<0.05）.Conclusion：Danshen Drink can play an anti-coronary atherosclerosis effect by mediating the PPAR-γ-LXR-α-ABCA1 signaling pathway.

Keywords Danshen Drink; Coronary atherosclerosis; PPAR-γ; ABCA1; LXR-α; Radix Salviae Miltiorrhizae; Lignum Santali Albi; Fructus Amomi Villosi; Prevention of coronary heart disease

中图分类号：R289.5;R541.4文献标识码：Adoi：10.3969/j.issn.1673-7202.2021.11.010

冠状动脉是动脉粥样硬化的主要累及血管，冠状动脉粥样硬化会导致患者出现管腔闭塞或破裂、出血，增加了患者的心血管疾病发生率，威胁到了患者的身体健康[1-3]。近年来，相关临床研究发现过氧化物酶体增殖物激活型受体γ（PPAR-γ）/肝X受体α（LXR-α）/三磷酸腺苷结合盒转运体A1（ABCA1）信号通路与机体的动脉粥样硬化密切相关[4]。其中PPAR-γ的活化可以减少机体内趋化因子受体的表达，从而减少了斑块周围产生的炎症反应，发挥出了对动脉粥样硬化的预防作用[5-6]。基于此，本研究丹参饮通过介导PPAR-γ-LXR-α-ABCA1信号通路减轻大鼠冠状动脉粥样硬化的作用机制。……