黄琳惠 丁秀秀 黄奕江 蔡兴俊

(海南省人民医院呼吸与危重症医学科 海南医学院附属海南医院，海南 海口 570311)

Effect of budesonide and Huaiqihuang on phagocytosis of macrophages OVA-induced asthmatic rats

HUANG Lin-Hui, DING Xiu-Xiu, HUANG Yi-Jiang,etal.

Department of Pulmonary and Critical Care Medicine, Hainan Province Hospital, Haikou 570311, Hainan, China

【Abstract】ObjectiveTo investigate the effect of budesonide and Huaiqihuang on phagocytosis of macrophages in OVA sensitive asthmatic rats.Methods60 male SD rats were selected and divided into budesonide group(n=12), control group (n=12), Huaiqihuang group (n=12), model group (n=12), and combination group (n=12) by the numerical expression method. The model of anaphylactic asthma was induced by OVA. After the last excitation, SD rats were executed, paraffin slices were taken from the upper lobe of the right lung, and HE staining was performed. The levels of immune function indexes such as phagocytosis of alveolar macrophages, immunoglobulin (Ig)E, interferon(IFN)-γ, interleukin(IL)-4 and IL-17 were compared.ResultsCompared with control group, the IFN-γ was significantly decreased and IL-4 and IL-17 were significantly increased in the model group (P=0.001). Compared with model group, the level of IFN-γ was increased in Huaiqihuang group (P=0.001). Compared with budesonide group and Huaiqihuang group, the level of IFN-γ was significantly increased and IL-17 was significantly decreased in the combination group (P=0.001). The phagocytosis of alveolar macrophages in model group was significantly lower than that of budesonide group and Huaiqihuang group(P=0.001). The phagocytosis of alveolar macrophages was significantly higher in combination group than that of Huaiqihuang group and budesonide group(P=0.001).There was positive relation between phagocytosis of macrophages and the IFN-α level of bronchoalveo lar lavage fluide(r=0.724,P=0.001).ConclusionsBudesonide and Huaiqihuang have positive effects on the phagocytosis of alveolar macrophages in rats, and could improve the status and immune function of asthmatic rats.

【Keywords】 Huaiqihuang; Budinide; Alveolar macrophages; Indicator of immune function

哮喘是临床上常见的支气管慢性炎症之一，其致病机制为支气管受外界抗原性刺激导致气道阻塞，引发慢性炎症〔1〕。哮喘患者的临床表现多数为可逆性气流受限。据相关文献报道，哮喘发病主要因Th细胞功能失衡引起。肺泡巨噬细胞是修复组织吞噬功能恢复的主要细胞。哮喘加重的主要因素为肺泡巨噬细胞吞噬功能受损〔2〕。布地奈德属于糖皮质激素的一种，可有效控制患者病情，但部分病例仍存在慢性进展和反复发作情况；槐杞黄主治益气养阴〔3〕。适用于气阴两虚引起的体质虚弱，可有效提升机体免疫力。目前，临床上对哮喘大鼠免疫机制的影响多数为单一槐杞黄或布地奈德实验，二者联合实验研究甚少〔4〕。本研究探讨布地奈德及槐杞黄对卵清蛋白(OVA)致敏哮喘型大鼠巨噬细胞吞噬功能的影响。

1 资料与方法

1.1实验动物 随机选取购自沈阳嘉乾程生物科技有限公司SPF级4～6周龄SD大鼠60只进行实验，运用随机数表法分为布地奈德组(n=12)、对照组(n=12)、槐杞黄组(n=12)、模型组(n=12)和联合组(n=12)。体重120～140 g。在50%湿度；23～25℃室温下饲养1 w。

1.2实验药品 布地奈德混悬液(国药准字：H20140475)规格：2 ml:1 mg×5支；AstraZeneca Pty Ltd公司生产。槐杞黄颗粒(国药准字：B20020074)规格：10 g×6袋/盒；启东盖天气药业公司生产。致敏混悬剂：OVA、氢氧化铝干粉各100 mg；5×109个灭火百日咳杆菌。

1.3哮喘模型建立 除对照组外4组大鼠进行建模，分别于实验第1天、第8天进行100 ml致敏混悬剂注射，实验第15天激发哮喘，每日上午8∶00,雾化吸入8 ml;1%OVA,1 d/次，连续7 d。……