外源ALA对板蓝根幼苗光合特性及叶绿素荧光参数的影响
2021-07-25张荣张文斌王勤礼陈修斌李文德张春梅李雨露
张荣 张文斌 王勤礼 陈修斌 李文德 张春梅 李雨露



摘要:以板蓝根品种安徽亳州种为试材,研究了不同浓度(200、400 mg/L)ALA对板蓝根幼苗光合特性和叶绿素荧光参数的影响。結果表明,与喷蒸馏水相比,板蓝根幼苗叶片的净光合速率(Pn),气孔导度(Gs),蒸腾速率(Tr)、胞间CO2浓度(Ci)显著提高,略提高了板蓝根水分利用率。200、400 mg/L ALA处理后,初始荧光(Fo)极显著(P < 0.01)降低,降低幅度分别为7.6%、14.7%;与喷蒸馏水相比,200 mg/L外源ALA处理最大荧光(Fm)、可变荧光(Fv)、最大光化学效率(Fv/Fm)、潜在光化学效率(Fv/Fo)、光能获取能力(1/Fo-1/Fm)分别提高1.06%、2.98%、1.97%、11.44%,10.98%;400 mg/L外源ALA处理分别提高1.72%,5.54%、3.81%、23.72%,23.53%,除(1/Fo-1/Fm)处理之间差异显著(P < 0.05)外,其余指标处理间差异均达极显著(P < 0.01)水平。喷施ALA能提高板蓝根幼苗植株的光合能力。
关键词:板蓝根;ALA;光合特性;叶绿素荧光参数
中图分类号:S567.2 文献标志码:A 文章编号:1001-1463(2021)06-0024-05
doi:10.3969/j.issn.1001-1463.2021.06.007
Effects of Exogenous ALA on Photosynthesis Characteristics and Chlorophyii Fluorescence Parameters of Radix Isatidis Seedlings
ZHANG Rong 1, ZHANG Wenbin 1, WANG Qinli 2, 3, CHEN Xiubin 2, 4, LI Wende 1, ZHANG Chunmei 2,4, LI Yulu 5
(1. Zhangye Economic Crops Technology Extending Stations, Zhangye Gansu 734000, China; 2. Key Laboratory of Hexi Corridor Characteristic Resources Utilization, Zhangye Gansu 734000, China; 3.Engineering & Technical Research Center for Greenhouse Vegetable in Hexi Corridor, Hexi University, Zhangye Gansu 734000, China; 4. College of Agriculture and Ecological Engineering, Hexi University, Zhangye Gansu 734000, China; 5. College of Earth Sciences, Chengdu University of Technology, Chengdu Sichuan 610059, China)
Abstract:Radix isatidis Anhui bozhou was used as the material, the effect of Exogenous ALA on Photosynthesis and chlorophyII fluorescence dynamics of radix isatidis(Isatis indigotica F.) seedlings was studied. The results showed that the net photosynthetic rate(Pn), stomatal conductance(GS), transpiration rate(Tr) and intercellular CO2 concentration(Ci) of seedling leaves of Radix isatidis were significantly increased compared with that of spraying distilled water, which slightly improved the water use efficiency of Radix isatidis. The initial fluorescence (Fo) was significantly decreased (P < 0.05) by 7.6% and 14.7% After 200 mg/L and 400 mg/L ALA treatment. The maximum fluorescence(Fm), the variable fluorescence(Fv.), the PS Ⅱmaximum photochemical efficiency(Fv/Fm), the potential photochemical efficiency(Fv/Fo) and the ability of PS Ⅱ reaction center to trap energy from antenna pigment(1/Fo-1/Fm) were increased by 1.06%, 2.98%, 1.97%, 11.44% and 10.98%, respectively, compared with spraying distilled water with 200 mg/L exogenic ALA;400 mg/L exogenous ALA treatment increased by 1.72%, 5.54%, 3.81%, 23.72%, 23.53%, respectively. There were significant differences among(1/Fo-1/Fm) treatments(P < 0.05), and the differences among other indexes were extremely significant(P < 0.01), which indicated that exogenous ALA treatment could improve photosynthesis ability of radix isatidis seedlings.
Key words:Radix isatidis;ALA;Photosynthesis;ChlorophyII fluorescence dynamics
光合作用是植物生物产量的主要决定因素之一,不同植物在不同环境下光合障碍的机理各不相同[1 - 4 ]。5-氨基乙酰丙酸(5- aminolevulinic acid,ALA)是所有生物体内卟啉化合物包括叶绿素、光敏素等生物合成的第一个关键前体[5 ]。在绿色植物中,ALA在质体中合成并转化为叶绿素和亚铁血红素,与植物的光合作用与呼吸作用关系密切。研究表明,无论是用ALA浸泡根系還是叶面喷施,其对植株整体都会产生生理效应[6 ]。板蓝根(Radix Isatidis)学名菘蓝(Isatis indigotica Fort.),又叫大青叶,十字花科二年生草本植物,用根入药称之为板蓝根,用叶入药称之为大青叶,具有清热解毒,凉血利咽之功效,是传统中药[7 - 10 ]。民乐县地处甘肃河西走廊中部,农区海拔1 589~ 5 027 m,全年无霜期140 d,境内土地肥沃,日照充足,气候温和,是典型的绿洲农业和培育天然绿色食品的理想之地。该区板蓝根常年种植面积稳定在10 000~16 700 hm2[11 ],素有“中国板蓝根之乡”的美称,种植板蓝根可以取得良好的经济效益与生态效益。板蓝根出苗后,个体绿色面积小,光合作用制造的养分也少,地上部、地下部都表现的生长缓慢。……
