杜丽娜 陈春帆 苏睿 谭春艳 赖彪

摘  要：为分析荔枝LcMYB1的功能，以白色‘W115矮牵牛和‘Micro-Tom番茄为材料，利用农杆菌介导法将LcMYB1在矮牵牛和番茄中异源表达，观测转基因植株表型。结果表明：与‘W115相比，转基因矮牵牛的叶片和花瓣中积累了花色苷，且矮牵牛花色苷生物合成结构基因PhCHS和PhDFR、调控基因PhAN1的表达显著上调;与野生型‘Micro-Tom番茄相比，转基因番茄的叶片和花药中积累了花色苷，且相应组织花色苷生物合成结构基因SlDFR、调控基因SlAN1和SlJAF13的表达显著上调，虽然果实中没有积累花色苷，但SlAN1和SlJAF13表达也显著上调。LcMYB1在矮牵牛和番茄中异源表达时通过上调花色苷生物合成关键结构基因和调控基因bHLH的表达诱导花色苷积累。因此，荔枝LcMYB1是花色苷生物合成中的关键转录因子，具备异源转化利用的潜力。

关键词：LcMYB1;矮牵牛;番茄;花色苷

中图分类号：S667.1      文献标识码：A

Abstract： In order to better understand the function of LcMYB1 and provide theoretical support for further utilization， LcMYB1 was transformed into both petunia and tomato. White flower ‘W115 petunia and ‘Micro-Tom tomato were used as materials in LcMYB1 ectopic expressed assays. Anthocyanin contents and related gene expressions were ana-lyzed in transgenic plants. Ectopic expression of LcMYB1 in ‘W115 resulted in anthocyanin production in vegetative and floral tissues such as leaves and petals， probably by transcriptional activation of anthocyanin biosynthetic genes such as PhCHS and PhDFR and endogenous anthocyanin regulatory gene PhAN1. However， the transgenic tomato only produced anthocyanin in anthers and leaves but not in tomato fruits and petals. The results suggested that LcMYB1 could enhance anthocyanin production in vegetative and floral tissues of both petunia and tomato by activating anthocyanin biosynthesis and regulatory genes. LcMYB1 is an important transcriptional regulatory factor in anthocyanin biosynthesis of plants and is potentially used in ectopic transformation.

Keywords： LcMYB1; petunia; tomato; anthocyanin

DOI： 10.3969/j.issn.1000-2561.2021.05.013

色澤是花卉和果蔬的重要品质组成之一。花色苷是植物组织或器官呈现出粉色、红色、紫色甚至黑色的重要次生代谢成分之一。花色苷除了能给蔬菜、花卉、水果带来鲜艳的颜色，还具有对生物和非生物胁迫给植物带来伤害的保护作用，比如：紫外线照射、冷胁迫、干旱胁迫等[1-3]。大量研究表明，花色苷合成代谢的调控主要在转录水平上[4]。转录因子R2R3-MYB、bHLH（basic Hlix-Loop-Hlix）和WD40相互作用形成MBW蛋白复合体，通过调控花色苷合成代谢的结构基因表达来调控植物花色苷的生物合成[5]。

苹果（Malus domestica）中有2个MYB转录因子调控果实花色苷的生物合成，其中MdMYB1主要调控光依赖的果皮着色[6]，而MdMYB10主要调控果肉的花色苷积累[7]。……