贾利强 赵秋芳 陈曙

摘  要：转录因子家族是由含有锌指结构的DOF结构域组成，构成植物特有的转录因子家族之一，在植物的生长发育进程中诸如信号转导、形态建成、抵御环境胁迫等方面都发挥着重要的作用。本研究利用定量PCR技术，探测了8个ZmDOFs基因在玉米6个不同组织的表达模式，结果表明，8个ZmDOFs基因具有不同的组织特异性表达模式，4个主要在幼穗中表达，2个主要在雄花中表达，ZmDOF28主要在苞叶中表达，而ZmDOF38在多种组织中表达，表明在玉米进化的进程中8个ZmDOFs基因表达模式的保守性和特异性。盐胁迫和干旱胁迫时，分别有7个和6个ZmDOFs基因受到明显的诱导，表明ZmDOFs基因廣泛参与玉米应答盐胁迫或干旱胁迫响应途径。在铵态氮胁迫和硝态氮胁迫时，分别有7个和8个ZmDOFs基因的表达受到了明显的改变，表明ZmDOFs基因参与玉米应答氮胁迫响应途径，体现了在其历史进化进程中的表达模式和功能的保守性与独特性。本研究为后续研究ZmDOFs基因的功能提供了参考。

关键词：DOF转录因子;进化树;组织表达;非生物胁迫

中图分类号：S513      文献标识码：A

Abstract： The DNA-binding one finger （DOF） transcription factor family is a major family of plant-specific transcrip-tion factors containing the DOF domain. These transcription factors are involved in a variety of functions of importance for different biological processes in plants. In the current study， an overview of 8 DOFs genes in maize is presented， including the gene structure， chromosome location， phylogeny， proten motif and expression pattern in responses to various abiotic stresses. Expression patterns indicated that the eight ZmDOFs had tissue specific expression patterns， indicating these genes are crucial in maize growth and development. Moreover， we examined the transcriptional levels of the eight ZmDOFs under salt and PEG6000 stress treatments， seven ZmDOFs and six ZmDOFs were up-regulated after salt and PEG6000 treatment， respectively. We also analyzed the eight ZmDOFs profilling under ammonium and nitrate stress treatment， seven and eight ZmDOFs significantly responded to these two stresses， indicating they are associated with nitrogen stresses responses. The results would provide a very useful reference for the cloning and functional analysis of the members of this gene family in maize and other species.

Keywords： DOF transcription factor; phylogenetic tree; tissue expression; abiotic stress

DOI： 10.3969/j.issn.1000-2561.2021.05.006

DOF蛋白是含有保守的DOF结构域的一类植物特有的转录因子。其保守的结构域一般位于DOF蛋白的N端，由保守的52个氨基酸组成的含有C2-C2锌指结构的元件组成，能特异性地结合下游基因启动子区域的保守元件5-T/AAAG-3来调控下游基因的表达[1]。DOF蛋白的C端一般有高度不保守具有调控基因表达的氨基酸序列组成[2]。第一个报道的DOF基因来自于玉米[3]，第一个报道DOFs基因家族的植物是拟南芥[4]。……