张敏 张雪松 刘雅萍

摘要 目的：建立雪蓮细胞培养物的质量标准。方法：参照《中华人民共和国药典》2020年版附录，分别建立雪莲细胞培养物的一般检查项目及定性定量指标。以芦丁为对照品，利用紫外分光光度法建立雪莲细胞培养物总黄酮成分含量的测定方法;利用高效液相色谱（HPLC）法同时测定绿原酸、紫丁香苷和1，5-二咖啡酰奎尼酸成分含量，并建立其特征图谱，采用2012版《中药色谱指纹图谱相似度评价系统》对特征图谱进行相似度分析。结果：15批次雪莲细胞培养物总黄酮类成分含量10.0%～11.0%;绿原酸含量0.86%～1.09%;紫丁香苷含量0.34%～0.48%;1，5-二咖啡酰奎尼酸含量3.20%～3.75%。15批雪莲细胞培养物指纹图谱相似度大于0.9，特征图谱中呈现10个特征峰，指认峰2为绿原酸、峰4为紫丁香苷、峰7为1，5-二咖啡酰奎尼酸。结论：本研究建立了雪莲细胞培养物的质量标准，建议醇溶性浸出物不得低于29.4%;雪莲细胞培养物中的总黄酮类成分含量不得低于10.0%，绿原酸、紫丁香苷、1，5-二咖啡酰奎尼酸的含量应分别不得低于0.86%、0.34%、3.20%，为天山雪莲药制品的工业化生产提供了参考。

关键词 雪莲细胞培养物;定性定量;总黄酮;绿原酸;紫丁香苷;1，5-二咖啡酰奎尼酸;特征图谱;质量标准

Abstract Objective：To establish the quality standard of Saussurea involucrata cell cultures.Methods：According to the appendix of Chinese Pharmacopoeia （2020 edition），the general examination items and qualitative and quantitative indexes of S.involucrata cell cultures were established.The content of total flavonoids in Saussurea involucrata cell culture was determined by ultraviolet spectrophotometry with rutin as the control substance; the content of chlorogenic acid，syringin and 1，5-dicaffeoylquinic acid was determined by HPLC simultaneously，and the characteristic chromatogram was established.The similarity of characteristic chromatogram was analyzed by the software Chinese Medicine Chromatographic Fingerprint Similarity Evaluation system（2012 edition）.Results：The total flavonoid content of 15 batches of S.involucrata cell cultures was between 10.0% and 11.0%; the content of chlorogenic acid was between 0.86% and 1.09%; the content of syringin was between 0.34% and 0.48%; 1，5-The content of dicaffeoylquinic acid was between 3.20% and 3.75%.The similarity of fingerprint patterns of 15 batches of S.involucrata cell cultures was greater than 0.9，and there were 10 characteristic peaks in the characteristic pattern.Peak 2 was chlorogenic acid，peak 4 was syringin，and peak 7 was 1，5-dicaffeoylquinic acid.Conclusion：In this study，the quality standard of S.involucrata cell cultures is established，and it is suggested that the alcohol soluble extract should not be less than 29.4%; the content of total flavonoids in S.involucrata cell cultures should not be less than 10.0%，and the content of chlorogenic acid，syringin and 1，5-dicaffeoylquinic acid should not be less than 0.86%，0.34% and 3.20%，respectively，which provides a reference for the industrial production of S.involucrata medicinal products.

Keywords Cell cultures of Saussurea involucrata; Qualitative and quantitative indicators; Total flavonoids; Chlorogenic acid; Syringin; 1，5-dicaffeoylquinic acid; Characteristic chromatogram; Quality standard

中图分类号：R284.1文献标识码：Adoi：10.3969/j.issn.1673-7202.2021.07.001

天山雪莲[Saussurea involucrate（Kar.et Kir.）Sch.Bip]又称雪莲、雪莲花、新疆雪莲，为菊科风毛菊属多年生草本植物，主要分布于新疆天山、昆仑山及阿尔泰山区终年积雪不化的地带，是一种高山稀有的名贵药用植物[1]。其入药始载于公元8世纪的藏药典《月王药诊》，并在之后多部著名医药典籍中出现，如《四部医典》《本草纲目拾遗》等，至今已有上千年的用药历史。……