李丹 石红 孔祥红 张景利

【摘 要】目的：探究类风湿关节炎患者外周血调节性T细胞（Treg细胞）分泌白细胞介素-35（IL-35）水平与其发病的相关性。方法：选取类风湿关节炎患者70例作为观察组，健康体检者70例为对照组。采集2组外周血，检测Treg细胞数量、Foxp3荧光强度、IL-35水平。同时将观察组患者按照28个关节疾病活动度评分（DAS28评分）分为不同的组，对各组Treg细胞、Foxp3荧光强度、IL-35水平进行检测。结果：观察组患者外周血中Treg细胞高于对照组，IL-35水平低于对照组，差异有统计学意义（P < 0.05）;

2组Foxp3荧光强度比较，差异无统计学意义（P > 0.05）。各DAS28评分组RA患者的Treg细胞、IL-35水平比较，差异有统计学意义（P < 0.05），且DAS28评分越高，Treg细胞数量和IL-35水平越低;各DAS28评分组RA患者Foxp3荧光强度比较，差异无统计学意义（P > 0.05）。结论：类风湿关节炎患者外周血中Treg细胞数量明显上升，IL-35水平显著下降。由于Treg细胞功能缺陷，降低了负调控能力。

【關键词】 类风湿关节炎;外周血调节性T细胞;白细胞介素-35;相关性

The Correlation Between the Level of IL-35 Secreted by Treg Cells in Peripheral Blood and the Pathogenesis of Rheumatoid Arthritis

LI Dan，SHI Hong，KONG Xiang-hong，ZHANG Jing-li

【ABSTRACT】Objective：To investigate the correlation between the level of IL-35 secreted by Treg cells in peripheral blood and the pathogenesis of rheumatoid arthritis（RA）.Methods：Seventy RA patients were selected as an observation group and 70 healthy physical examinees were selected as a control group.The peripheral blood of both groups was collected.The number of Treg cells，the fluorescence intensity of Foxp3 and the level of IL-35 were measured.At the same time，the patients in the observation group were divided into different groups according to the DAS28 score，and the Treg cells，Foxp3 fluorescence intensity and IL-35 levels in each group were detected.Results：The level of Treg cells in peripheral blood of the observation group was higher than that of the control group，and the level of IL-35 was lower than that of the control group（P < 0.05）;There was no significant difference in the fluorescence intensity of Foxp3 between the two groups（P > 0.05）.The difference of Treg cells and IL-35 levels in RA patients with different DAS28 scores was statistically significant（P < 0.05），and the higher the score of DAS28，the lower the number of Treg cells and IL-35 levels;There was no significant difference in the fluorescence intensity of Foxp3 between RA groups with different DAS28 scores（P > 0.05）.Conclusion：The number of Treg cells in the peripheral blood of RA patients increased significantly，and the level of IL-35 decreased significantly.Because of the deficiency of

Treg cells，the negative regulation ability was reduced.

【Keywords】 rheumatoid arthritis;

Treg cells of peripheral blood;IL-35;correlation

类风湿关节炎（rheumatoid arthritis，RA）为常见的全身性自身免疫性疾病，主要表现为慢性侵蚀性关节炎。RA患者往往出现关节肿痛、滑膜关节破坏，严重影响患者的正常生活。RA是造成劳动力丧失和致残的一个重要原因。目前，RA的发病机制尚不明确[1-2]。其中，CD4+T细胞亚群失衡、炎症介质异常表达等，对RA发病有重要的作

用[3-4]。CD4+CD25+调节性T细胞（Treg细胞）的两大功能，包括免疫无反应性和免疫应答负调节。CD4+CD25+Treg细胞发挥负调控的作用，对RA的发病和进展产生影响。白细胞介素-35（IL-35）主要由Treg细胞分泌，发挥必需的抑制作用，促使发挥Treg细胞最大抑制效应。IL-35表达缺失，一定程度上影响Treg细胞的抑制功能。本文探究RA患者Treg细胞分泌IL-35水平，及与其发病的相关性，现总结报道如下。