李吉庆 林道斌 张永杰

摘要 目的：运用网络药理学研究方法对黄连-葛根药对治疗2型糖尿病（T2DM）的作用机制进行分析。方法：采用中药系统药理学分析平台（Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform，TCMSP）筛选出黄连-葛根（Coptis Chinensis-Pueraria Lobata，C-P）药对的有效活性成分及作用靶点蛋白，再使用Unipro数据库将筛选出的靶点蛋白转换为基因名，通过GeneCards数据库收集T2DM疾病基因，然后对药物作用基因及疾病相关基因进行韦恩（Venn）分析，寻找交集靶点，用交集靶点与对应活性成分构建活性成分-靶点相互作用网络，并对交集基因进行GO功能富集分析和KEGG通路富集分析。结果：研究得到13个作用于T2DM疾病靶点的活性成分和146个作用靶点，GO功能富集分析确定了284个条目，KEGG通路分析共发现77条作用通路。结论：本研究结果初步探讨了C-P药对治疗T2MD的基本药理作用及其机制，并为进一步的试验研究奠定了良好的基础。

关键词 网络药理学;黄连;葛根;2型糖尿病;作用机制;前列腺素G/H合酶1;前列腺素G/H合酶2;PI3K-Akt信号通路

Mechanisms of the Drug Pair Coptis Chinensis-Pueraria Lobata in the Treatment of Type 2 Diabetes Mellitus Based on Network Pharmacology

LI Jiqing，LIN Daobin，ZHANG Yongjie

（Department of Endocrine，Chinese Medicine Hospital of Hainan Province，Haikou 570203，China）

Abstract Objective：To analyze the mechanism of the drug pair Coptis chinensis-Pueraria lobata in the treatment of type 2 diabetes mellitus（T2MD）by using the method of network pharmacology.Methods：The active components and target proteins of the drug pair Coptis chinensis-Pueraria lobata（C-P）were screened by Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform（TCMSP），and the target proteins were screened by Unipro database and converted into gene names.The disease gene of T2MD was collected through GeneCards database.Then the drug-acting genes and disease-related genes were analyzed by vnne to find the intersecting targets，with which plus their corresponding active components an active component-target interaction network was constructed.GO function enrichment analysis and KEGG pathway enrichment analysis of the intersecting genes were also carried out.Results：A total of 13 active components acting on T2MD and 146 effect targets were identified，284 items were identified by GO functional enrichment analysis，and 77 pathways were identified by KEGG pathway analysis.Conclusion：The results of this study preliminarily discussed the basic pharmacological effects and mechanisms of C-P drugs on the treatment of T2MD，and laid a good foundation for further experimental research.

Keywords Network pharmacology; Coptis chinensis; Pueraria lobata; Type 2 diabetes; Mechanism of action; Prostaglandin G / H synthase 1; Prostaglandin G / H synthase 2; PI3K Akt signaling pathway

中图分类号：R282;R587.1文献标识码：Adoi：10.3969/j.issn.1673-7202.2021.06.007

作为一种常见的慢性疾病，糖尿病在2015年的全球发病已经超过4.15亿，预计在未来30年将达到6.42亿[1]。在我国，糖尿病与心血管病以及肿瘤，已成为威胁人民健康的3大慢性病。在过去的30年里，经过7次全国性调查发现，中国内地的糖尿病患者增长了17倍，其中2型糖尿病患者占比高达90%～95%[2]。所以针对糖尿病，特别是2型糖尿病的药物开发已经日益迫切。最近，由于同時具有安全性、低成本和有效性，中药治疗糖尿病受到了广泛关注[3]，C-P药对就是其中的代表性药物之一。……