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利用高通量表型平台分析紫叶紫菜薹新组合19-520的表型特征

2021-06-30朱红芳李晓锋奚丹丹高璐张兆辉朱玉英

江苏农业学报 2021年2期

朱红芳 李晓锋 奚丹丹 高璐 张兆辉 朱玉英

摘要: 以紫叶紫菜薹组合19-520和2个亲本材料为试验材料,于播种后75 d,以普通绿叶紫菜薹为对照,采用高通量表型平台进行表型测定和LemnaTec Scanalyzer 3D成像。研究了紫叶紫菜薹组合19-520及其亲本株高、幅宽、紧密度、投影面积和植株相对含水量等指标的差异。结果表明,紫叶紫菜薹组合19-520的株高、幅宽、植株紧密度、投影面积、相对含水量和花青素相对含量均较高,表型特征表现优良。因此,紫叶紫菜薹组合19-520作为新的紫菜薹组合在综合表型上表现良好,可进一步推广应用。

关键词: 紫菜薹;株高; 投影面积;紧密度;相对含水量

中图分类号: S634.6  文献标识码: A  文章编号: 1000-4440(2021)02-0465-06

Abstract: The combination of 19-520 and its two parent materials were used as the test meterials and the normal green leaf purple-caitai was used as the control to test the phenotype and make the LemnaTec Scanalyzer 3D imaging by using the high-throughput phenotype platform 75 days after sowing. The differences in plant height, width, compactness, projection area and relative water content of the combination 19-520 and its parents were studied. The results showed that the plant height, width, plant compactness, projection area, relative contents of water and anthocyanin of 19-520 were all better than its parents and the normal green leaf purple-caitai, and the phenotypic characteristics were excellent. As a new purple leaf purple-caitai combination,19-520 has significant comprehensive phenotypic characteristics, which can be further applied in popularization and application.

Key words: purple-caitai;plant height;projection area;compactness;relative water content

植物遗传学、育种学和生物信息学的研究离不开植物基因型、表型和环境这3个因素[1],生物学家Freimer等[2]、Houle等[3]和Gjuvsland等[4]先后对表型进行了定义,认为表型是生物某一特定的物理外观或者结构组成,也就是植株个体在特定环境条件下表现出来的性状特征总和。研究生物表型特征的学科被称为表型组学,这一概念由Steven A.Garan在1996年最早提出,他将生物各方面的表型特征作为一个整体来研究,这个研究的整体即为表型组学。将生物群体详细的表型数据信息结合生物群体的基因型特征进行分析,是基因组学研究的基础,并可以应用于复杂生物体生命系统的研究中[5]。利用可见光、超光谱、近红外、远红外以及X光成像技术构建分析平台,并利用该平台进行表型分析的组学称为高通量表型组学,利用高通量表型组学可以在短时间内实现大量植物个体多维表现型的高精度获取和定量分析现代技术体系[6]。……

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