国佳鑫 侯丽 蔡和平 栗枭杰 杨茜茹 张思亲 董青

摘要 目的：通過网络药理学方法探讨新加良附方治疗胃癌的作用机制。方法：在中药系统药理学数据库与分析平台（TCMSP）中获取高良姜、香附、穿山龙的有效成分及靶蛋白，通过Genecards、OMIM数据库筛选出胃癌的靶点，利用Venn软件获取共同靶点，使用Cytoscape3.7.2进行“化合物-靶点-疾病”的网络图构建。通过String数据库获取蛋白互作网络（PPI），最后进行GO功能、KEGG通路富集分析。结果：从新加良附方中筛选出高良姜素、槲皮素、木犀草素、薯蓣皂苷元等主要有效活性成分，作用于87个胃癌靶点，筛选出AKT1、MYC、CASP3、EGFR等关键靶点，并确定了PI3K-Akt信号通路、p53信号通路、Wnt信号通路等关键代谢信号通路，参与蛋白结合、转录因子活性、酶活性调节、受体活性等过程。结论：新加良附方通过多个靶点、多条通路发挥治疗胃癌的作用。

关键词 新加良附方;寒凝血瘀;高良姜;香附;穿山龙;胃癌;网络药理学;作用机制

Based on the Key Pathogenesis Theory of “Cold Coagulation and Blood Stasis” Discussing the Network Pharmacology Study of Modified Liang-Fu Formula In The Treatment of Gastric Cancer

GUO Jiaxin1，HOU Li1，CAI Heping2，LI Xiaojie1，YANG Qianru1，ZHANG Siqin3，DONG Qing1

（1 Dongzhimen Hospital，Beijing University of Chinese Medicine，Beijing 100020，China; 2 Beijing Traditional Chinese Medicine Hospital Shunyi Hospital，Beijing 101399，China; 3 Hospital of Chengdu University of Traditional Chinese Medicine，Chengdu 610075，China）

Abstract Objective：To explore the mechanism of action of modified Liang-Fu Formula in the treatment of gastric cancer through network pharmacology method.Methods：The effective components and target proteins of Alpinia officinarum Hance，Cyperus rotundus L and Discorea nipponica Makino were from the traditional Chinese medicine system pharmacology analysis platform （TCMSP）.And the target of gastric cancer was gotten through the Genecards and OMIM database.Venn online software was used to obtain the common target，and the “compound-target-disease” network diagram was constructed by using Cytoscape 3.7.2 software.The String database was used to draw the protein interaction（PPI） network.Finally，enrichment analysis of GO function and KEGG pathway was carried out.Results：The main active components，such as galangin，quercetin，luteolin and diosgenin，were screened out from the negaeliang side，and acted on 87 gastric cancer targets.Key targets，such as AKT1，MYC，CASP3 and EGFR，were screened out，and key metabolic signaling pathways，such as pi3K-Akt signaling pathway，p53 signaling pathway，and Wnt signaling pathway，were determined.It was involved in protein binding，transcription factor activity，enzyme activity regulation，receptor activity and other processes.Conclusion：Modified Liangfu Formula plays a role in the treatment of gastric cancer through multiple targets and pathways.

Keywords Modified Liang-Fu Formula; Cold coagulation and blood stasis; Alpinia officinarum Hance; Cyperus rotundus L; Discorea nipponica Makino; Gastric cancer; network pharmacology; Mechanism of action

中图分类号：R285文献标识码：Adoi：10.3969/j.issn.1673-7202.2021.03.004

在世界范围内，亚洲为胃癌发病率最高的地区。据统计，2015年中国恶性肿瘤新发病例42.92万例，死亡病例28.14万例，而胃癌的发病率和病死率分别为679.1/10万和498/10万[1]。尽管近年来胃癌的发病率及病死率均有所下降，但胃癌患者的治疗及其由此产生的不良反应仍是困扰医学界的一大难题。中医药在改善胃癌患者症状、延迟生存期方面具有独特的优势，有待进一步研究。

网络药理学是通过对生物系统的网络分析，选取特定信号节点进行多靶点药物分子分析的新方法[2]。……