余绍蕾 徐洪霞 黄少梅

【摘 要】 目的：观察抗氧化贴剂对UV照射造成的皮肤损伤的修复作用。方法：建立家兔UV照射皮肤损伤模型，治疗组于UV照射后分别敷贴抗氧化贴剂不同的时间，连续辐照、敷贴30d，观察皮肤变化情况，检测皮肤中SOD、CAT和MDA的含量。结果：治疗组红斑、水肿、出血、渗出症状较模型组出现得晚，且症状轻，治疗组与模型组比较，症状评分均有明显改善（P<0.01）。治疗组与模型组比较，SOD和CAT含量明显升高， MDA含量明显降低（P<0.01）。结论：抗氧化凝胶贴剂对UV照射造成的皮肤有一定的修复作用。

【关键词】 抗氧化贴剂;紫外线;皮肤损伤

Abstract：Objective To observe the repairing effect of Antioxidant Patch on skin damage caused by UV irradiation.Methods The model of rabbit skin injury induced by UV irradiation was established.The treatment group was treated with Antioxidant Patch for 30 days after UV irradiation.The changes of skin were observed and the contents of SOD，CAT and MDA in the skin were detected.Results The symptoms of erythema，edema，bleeding and exudation in the treatment group appeared later than that in the model group，and the symptoms were mild.The symptom scores of the treatment group and the model group were significantly improved，P<0.01.Compared with the model group，the content of SOD and CAT in the treatment group was significantly increased，while the content of MDA was significantly decreased （P< 0.01）.Conclusion The Antioxidant Patch has certain repair effect on the skin damage caused by UV irradiation.

Keywords：Antioxidant Patch;UV;Skin Damage

紫外線（Ultravioletray，UV）在自然界中无处不在，其对皮肤的损伤已有大量的研究。皮肤长期受紫外线照射，会造成积累性的损伤，表现为皮肤变黑、色斑、皮肤松弛、皱纹加深;严重的导致局部红肿、出血、炎症反应等。其作用机制之一是紫外线使人体皮肤中活性氧自由基含量急剧升高，进而会破坏正常组织细胞的大分子物质代谢，导致蛋白质、脂类和DNA的氧化性损伤[1]。

抗氧化水凝胶贴剂是根据UV照射造成皮肤损伤的机理，以缓解临床症状、修复损伤皮肤为目标，经科学的方法筛选、优化形成的配方，包括海藻糖、透明质酸钠、芦荟提取物、积雪草提取物、螺旋藻多糖等原料。制成水凝胶贴剂具有保湿性强、载药量大、剂量准确、清爽不油腻、使用方便等优势，适合于皮肤局部使用。在对配方筛选、质量标准研究的基础上，对抗氧化水凝胶贴剂的功效和作用机理也进行了研究，以进一步验证配方的可行性。

1 材料与方法

1.1 动物 30只健康成年白色新西兰家兔由广东省实验动物中心提供[动物生产许可证号：SCXK（粤）2019-0035;动物合格证号：44116000003 54]， 体重2.0～2.5kg，无皮肤疾病史，雌雄不限。……