黄杰中 陈敬恒

【摘要】 目的：探究c-myc蛋白及凋亡抑制、促進因子在骨肉瘤中的检测价值。方法：选取2017年1月-2019年7月于本院就诊的60例骨肉瘤患者为观察组，同时期的60例骨良性肿瘤患者为对照组。检测并比较两组的组织c-myc蛋白及凋亡抑制因子（Bcl-2、NF-κB、Fas及Livin）、凋亡促进因子（Caspase-3、Caspase-8及Bax）表达情况，同时比较观察组中不同分化程度者的上述指标表达情况，分析上述指标与骨肉瘤分化程度的关系。结果：观察组的组织c-myc蛋白及凋亡抑制因子表达均显著高于对照组，组织凋亡促进因子均显著低于对照组;且观察组中肿瘤低分化者组织c-myc蛋白表达均显著高于高、中分化者，组织凋亡促进因子均显著低于高、中分化者，组织凋亡抑制因子表达均显著高于高、中分化者;肿瘤中分化者组织c-myc蛋白表达均显著高于高分化者，组织凋亡促进因子均显著低于高分化者，组织凋亡抑制因子表达显著均高于高分化者，差异均有统计学意义（P<0.05）。Spearman秩相关分析显示，c-myc蛋白及凋亡抑制因子与骨肉瘤分化程度呈负相关（P<0.05），组织凋亡促进因子与骨肉瘤分化程度呈正相关（P<0.05）。结论：c-myc蛋白及凋亡抑制、促进因子在骨肉瘤中的表达显著异常，且与骨肉瘤分化程度密切相关，因此在骨肉瘤患者中的检测价值较高。

【关键词】 c-myc蛋白 凋亡抑制因子 凋亡促进因子 骨肉瘤

[Abstract] Objective： To investigate the detection value of c-myc protein and apoptosis inhibition，promotion factors of patients with osteosarcoma. Method： A total of 60 patients with osteosarcoma from January 2017 to July 2019 were selected as the observation group， 60 patients with benign bone tumor during the same period were selected as the control group. The expressions of c-myc protein and apoptotic inhibitor factors （Bcl-2， NF-B， Fas and Livin） and apoptotic promotion factors （Caspase-3， Caspase-8 and Bax） in the two groups were detected and compared. Meanwhile， the expressions of the above indexes in the observation group were compared with different grade of differentiation， analyze the relationship between the above indexes and the degree of differentiation of osteosarcoma. Result： The expression of c-myc protein and apoptosis inhibition factors in the observation group were significantly higher than those in the control group， while the expression of tissue apoptosis promoting factor was significantly lower than that in the control group; in the observation group， the expression of c-myc protein were significantly higher in the poorly differentiated group than in the highly and moderately differentiated groups， the expression of tissue apoptosis promotion factors were significantly lower than those in the highly and moderately differentiated groups， the expression of tissue apoptosis inhibition factors were significantly higher than those in the highly and moderately differentiated groups; the expression of c-myc protein in moderately differentiated tumors was significantly higher than that in highly differentiated tumors， the expression of tissue apoptosis promoting factor was significantly lower than that in highly differentiated tumors， the expression of tissue apoptosis inhibiting factor was significantly higher than that in highly differentiated tumors， the differences were statistical significance （P<0.05）. Spearman rank correlation analysis showed c-myc protein and apoptosis inhibition factors were negatively correlated with differentiation degree of osteosarcoma （P<0.05）， the tissue apoptosis promotion factors were positively correlated with differentiation degree of osteosarcoma （P<0.05）. Conclusion： The expression of c-myc protein and apoptosis inhibition，promotion factors of patients with osteosarcoma are significantly abnormal， they are close correlated with differentiation degree of osteosarcoma， so their detection value in the patients with osteosarcoma are higher.

[Key words] c-myc protein Apoptosis inhibition factors Apoptosis promotion factors Osteosarcoma

First-authors address： Panyu District Hospital of Traditional Chinese Medicine， Guangzhou 511400， China

doi：10.3969/j.issn.1674-4985.2021.09.034

骨肉瘤是临床并不乏见的一类恶性肿瘤，与本病相关的研究中，细胞凋亡相关因子的研究可见，但是针对性的凋亡抑制、促进因子的研究则相对不足，且现存的研究差异显著，与骨肉瘤分化程度的研究极为匮乏，因此上述凋亡相关因子与骨肉瘤的细致关系研究仍有待深入探究[1-2]。同时，c-myc蛋白作为可促进细胞无限增殖的指标，其在促进细胞分裂中的作用较强，其在骨肉瘤中的表达研究也可见[3-5]，但是其同样存在在骨肉瘤患者中研究细致程度不足的情况。……