孙娟　林晖　王耀新　蔡惠美　唐晶晶

【摘要】 目的：研究與分析PYCR1在胃癌细胞中的表达及生物学功能。方法：选取2017年10月-

2020年1月本院32例胃癌患者为观察组，同时期的32例胃炎患者为对照组。比较两组的病灶组织PYCR1表达情况，并比较观察组中不同疾病分期及淋巴结转移情况者的PYCR1表达情况，采用Spearman秩相关分析其与疾病分期及淋巴结转移情况的关系;取胃癌AGS、SGC-790及MGC-803细胞分别分为si-NC组（转染对照质粒）与si-PYCR1组（转染敲低序列PYCR1质粒），比较各组的细胞克隆数、凋亡细胞率及细胞穿膜数。结果：观察组的PYCR1表达水平高于对照组（P<0.05）。观察组中不同疾病分期及淋巴结转移情况者的PYCR1表达水平比较，差异均有统计学意义（P<0.05），Spearman秩相关分析显示，PYCR1与疾病分期及淋巴结转移情况均呈正相关（rs=0.910、0.881，P<0.05）。AGS、SGC-790及MGC-803细胞的si-PYCR1组的细胞克隆数及细胞穿膜数均低于si-NC组，凋亡细胞率均高于si-NC组，差异均有统计学意义（P<0.05）。结论：PYCR1在胃癌细胞中呈现高表达，且与疾病分期及淋巴结转移情况均呈正相关，干扰PYCR1可有效控制胃癌细胞增殖及迁移侵袭。

【关键词】 PYCR1 胃癌细胞 增殖 迁移 侵袭

Study on the Expression of PYCR1 in Gastric Cancer Cells and Biological Function/SUN Juan， LIN Hui， WANG Yaoxin， CAI Huimei， TANG Jingjing. //Medical Innovation of China， 2021， 18（23）： -166

[Abstract] Objective： To study and analyze the expression and biological function of PYCR1 in gastric cancer cells. Method： From October 2017 to January 2020， 32 patients with gastric cancer in our hospital were selected as the observation group， and 32 patients with gastritis during the same period were selected as the control group. The expression of PYCR1 in the two groups was compared， and the expressions of PYCR1 in patients with different disease stages and lymph node metastasis in the observation group were compared. The relationship between PYCR1 and disease stage and lymph node metastasis was analyzed by Spearman rank correlation. Gastric cancer AGS， SGC-790 and MGC-803 cells were divided into si-NC group （transfected control plasmid） and si-PYCR1 group （transfected knockdown sequence PYCR1 plasmid）， respectively. The number of cell clones， apoptotic cell rate and cell transmembrane number were compared in each group. Result： The expression level of PYCR1 in the observation group was higher than that in the control group （P<0.05）. The differences of PYCR1 expression levels in patients with different disease stages and lymph node metastasis in the observation group were statistically significant （P<0.05）. Spearman rank correlation analysis showed that， PYCR1 was positively correlated with disease stage and lymph node metastasis （rs=0.910， 0.881， P<0.05）. The number of cell clones and cell transmembrane number in si-PYCR1 group of AGS， SGC-790 and MGC-803 cells were lower than those in si-NC group， and the rates of apoptotic cells were higher than those in si-NC group， the differences were statistically significant （P<0.05）. Conclusion： PYCR1 is highly expressed in gastric cancer cells and is positively correlated with disease stage and lymph node metastasis， interfering with PYCR1 can effectively control the proliferation， migration and invasion of gastric cancer cells.

[Key words] PYCR1 Gastric cancer cells Proliferation Migration Invasion

First-author’s address： Fuzhou First Hospital， Fuzhou 350009， China

doi：10.3969/j.issn.1674-4985.2021.23.040

胃癌是消化系统常见恶性肿瘤，与胃癌相关的各方面研究一直是临床重点。与胃癌预后相关的研究中，胃癌细胞的增殖侵袭及转移能力是重要因素[1-2]。PYCR1作为在多类恶性肿瘤患者中研究较多的指标，较多研究认为其对于肿瘤细胞的增殖及侵袭转移具有促进作用[3-5]。临床中关于PYCR1在胃癌细胞中的作用机制，尤其是生物学功能的研究极为匮乏，且在细致程度方面有所欠缺，因此本方面的研究意义较高。……