申小年 彭俊宇 戴俭华

摘  要：目的  分别用NaV1.7离子通道测试的常用中药的抑制率，比较这些常用药物的疗效，为临床处方治疗疼痛提供理论指导。方法  选择三七、天麻、全蝎、蘄蛇各20 g，根据2015版中药药典通则2201的40 g对应1 000 mL水的方法，冷凝水煎提取。然后过0.22 μm滤膜，使用全细胞膜片钳技术，研究这些药物分别对NaV1.7离子通道抑制作用。结果  抑制率的比较的结果：三七的抑制率最高，临床有统计学意义。结论 对于临床骨科常用药物止痛效果有一定的指导意义。

关键词：疼痛  膜片钳  离子通道  中药镇痛

中图分类号：R73            文献标识码：A               文章编号：1672-3791（2021）01（a）-0250-03

Comparison of Inhibition Rates of Different Traditional Chinese Medicine on Nav1.7 Ion Channel

SHEN Xiaonian1  PENG Junyu2  DAI Jianhua2

（1.Anhui College of Traditional Chinese Medicine; 2. Anhui Wuhu Hospital of Traditional Chinese Medicine Affiliated to Anhui College of Traditional Chinese Medicine， Wuhu， Anhui Province， 241000 China）

Abstract： Objective The inhibition rates of commonly used traditional Chinese medicines tested with NaV1.7 ion channels were compared， and the therapeutic effects of these commonly used medicines were compared to provide theoretical guidance for the clinical prescription of pain. Methods 20 g each of Panax notoginseng， Gastrodia elata， whole scorpion， and cypris were selected. According to the method of 2015 edition of the General Principles of Chinese Medicine Pharmacopoeia 2201， 40 g corresponded to 1 000 mL of water，condensate decoction extraction. Then through a 0.22 μm filter， using whole-cell patch-clamp technology to study the inhibitory effects of these drugs on NaV1.7 ion channels. Results  The result of the comparison of the inhibition rate： Panax notoginseng has the highest inhibition rate， which is statistically significant. Conclusion It has certain guiding significance for the analgesic effect of commonly used drugs in clinical orthopedics.

Key Words： Pain; Patch clamp; Ion channel; Analgesia with traditional Chinese medicine

疼痛是一种在躯体或内脏组织中产生的具有潜在伤害性或受到有害刺激之后的令人不快的感觉。炎症和神经性疼痛与急性疼痛不同，如关节炎和偏头痛，在大部分人身上往往表现出的是持久的慢性状态，医疗保健费用花费达数十亿美金并且造成生产力损失[1]。钠离子通道的表达异常可能与炎性痛、神经病理性痛和癌痛等多种慢性痛的发病机制相关[2]。电压门控钠通道是完整的跨膜蛋白，为兴奋性细胞的快速去极化提供电流通路，并且它们在传递伤害感受器对背角突触的反应中起关键作用[3]。NaV1.7离子通道主要集中分布于感觉神经的背根神经节和交感神经的神经节中，同时也分布于伤害性刺激密切相关的传入神经纤维Aβ纤维和C纤维中表达[4]。

近年来，基于几项显著的人类遗传学研究，NaV1.7已成为一种极有研究价值的镇痛靶点。……