刘雪梅，王悦，李玮琦，魏继承，任如意，郝爱平

摘要：为了解miR169基因家族在辣椒生长发育过程的重要调控作用，通过生物信息学的方法对can-miR169基因家族的成员进行了染色体定位、序列保守性分析、系统发育进化树分析、前体序列二级结构预测和靶基因预测。结果表明：can-miR169家族成员定位在4条染色体上;成熟序列高度保守，前体序列在产生成熟序列的位置高度保守;预测结果显示can-miR169家族成员均有靶基因，这些靶基因主要以靶基因核因子A（NF-YA）复合物为主，且大体相同。这些靶基因可以参与到种子萌发、植株发育和对外界环境胁迫的应答等多种生命活动中。

关键词：miR169;辣椒;生物信息学;NF-YA

中图分类号：S641.3文献标识码：A文章编号：1000-4440（2021）06-1510-06

Bioinformatics analysis of miR169 and its target genes in pepper

LIU Xue-mei，WANG Yue，LI Wei-qi，WEI Ji-cheng，REN Ru-yi，HAO Ai-ping

（ College of Life Sciences and Technology， Mudanjiang Normal College， Mudanjiang 157011， China）

Abstract： In order to understand the important regulatory role of miR169 gene family in the growth and development of pepper， the members of can-miR169 gene family were analyzed by bioinformatics， including chromosome mapping， sequence conservation analysis， phylogenetic tree analysis， precursor sequence secondary structure prediction and target gene prediction. The results showed that the members of can-miR169 family were located on four chromosomes， the mature sequence was highly conserved. The precursor sequence was highly conserved in the position where the mature sequence was produced. The forecasting results indicated that all members of can-miR169 family had target genes， which were mainly composed of nuclear factor Y （NF-YA） complexes and were roughly the same. These target genes can be involved in a variety of life activities such as seed germination， plant development and response to external environmental stress.

Key words：miR169;pepper;bioinformatics;NF-YA

MicroRNA（miRNA）是真核生物体中内生的，长度大致为20～24个核苷酸[1]，具有一定的保守性、时序性和结构特异性的非编码小分子RNA。miRNA调控植物的生长发育、信号转导和对外界环境非生物胁迫的响应等生命活动[2]。一个miRNA可能具有一个或多个靶基因，且miRNA一般都是通过对靶基因的切割和翻译抑制[3]对靶基因进行调控。

从目前的研究结果来看，miRNA家族庞大且调控方式复杂，其中，miR169是植物miRNA中几个最大的miRNA家族之一，仅水稻miR169家族就登记了十几个家族成员，且成员间具有大体相同的成熟序列和靶基因。近年，越来越多的科研人员研究了miR169对植物的逆境调控作用。Li等[4]发现miR169可在水稻抗稻瘟病中起负调节作用。陈禹彤等[5]发现miR169o可以参与干旱胁迫的应答。舒李露等[6]发现当miR169过表达时能够促进山核桃植株提前开花。……